锂-毛果芸香碱致大鼠早期大脑NG2和O4表达及意义  

Early Phase Expression of NG2 and O4 in the Brain of Epileptic Rats Induced by Chloride Lithium-pilocarpine

作　　者：李华杰[1] 包仕尧[2] 张志琳[2] 吴莹[2] 

机构地区：[1]常州市第一人民医院神经内科,213000 [2]苏州大学附属第二医院神经内科,215000

出　　处：《中国临床神经科学》2004年第4期354-357,共4页Chinese Journal of Clinical Neurosciences

摘　　要：目的 :探讨氯化锂 毛果芸香碱 (匹罗卡品 )致疒间大鼠早期大脑少突胶质前体细胞变化及意义。方法 :对雄性成年SD大鼠先后腹腔注射氯化锂、毛果芸香碱 ,制成癫疒间持续状态动物模型 ;用免疫荧光组织化学法检测疒间 性发作后早期大鼠大脑皮质和海马CA1区NG2和O4阳性细胞数量。结果 :和对照组相比 ,除癫疒间后 1d组外其余各组大鼠脑皮质内NG2和O4阳性细胞都有明显的增加 ;癫疒间后 1d组海马CA1区的阳性细胞数明显减少 ;癫疒间 后 7d组皮质和海马CA1区NG2和O4阳性细胞数最多。结论 :氯化锂 毛果芸香碱致疒间 大鼠早期大脑NG2和O4表达增加 ,少突胶质前体细胞增多 ,并且和观测时间相关。Aim:To explore the early phase change of oligodendrocyte precursor cells in the brain of epileptic rats induced by chloride lithium-pilocarpine.Methods:To establish status epilepsy model of male adult SD rats by intraperitoneal injection with chloride lithium and pilocarpine.Then to determinate NG2 or O4-positive cells in the cortex and hippocampal field CA1 of epilptic rats at each time-point by immunofluorescence histochemistry.Results:The number of NG2 and O4-positive cells within the cortex of epileptic rats increases significantly at each time-point except for 1 day after seizure comparing with the control group.The number of NG2 and O4-positive cells o hippocampal field CA1 decreases significantly at the time-point except for 1 day after seizure comparing with the control group.The number of NG2 and O4-positive cells o hippocampal field CA1 decrease significantly at the time-point of 1 day after seizure.The number of NG2 and O4-positive cells in the cortex and hippocampal field CA1 at 7 days after epilepsy has the most significant increase in all groups.Conclusion:The early phase expression of NG2 and O4 and the number of oligodendrocyte precursor cells in the brain of epileptic rats increase and relate to the observing time-point.

关 键 词：毛果芸香碱 NG 致痫大鼠 早期 阳性细胞 海马CA1区 大脑 氯化锂 雄性 前体细胞 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R759[医药卫生—临床医学]