表达NDV F48E9株F基因的MDV CVI988株转移质粒载体的构建  

Construction of recombinant Marek's disease virus CVI 988 strain transferring plasmid vector expressingF gene of NDV F48E9 strain

作　　者：苏春霞[1] 张彦明[1] 张雪莲[2] 徐学清[2] 陈溥言[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]南京农业大学农业部畜禽疫病诊断与免疫重点开放实验室,江苏南京210095

出　　处：《西北农林科技大学学报（自然科学版）》2005年第1期1-4,共4页Journal of Northwest A&F University(Natural Science Edition)

摘　　要：　将新城疫病毒(NDV)F48E9株融合蛋白(F)基因1700bp克隆到真核表达载体pIRES中,构建成表达F基因的载体pIRESF,然后将包含F基因及其上游的内含子和下游的polyA的2900bpDNA片段再克隆入包含gB启动子的SK载体中,并使其克隆到gB启动子600bp的下游,最后将包含gB启动子和F基因表达盒3500bp克隆到包含MDVCVI988非必须片段US10的载体SK中。该研究为进一步在细胞中转染并获得表达F基因的MDVCVI988重组病毒奠定了基础。The NDV F48E9 strain fusion protein gene was cloned into the multi clone site of eukaryotic expression vector pIRES to form PIRESF.Then 2 900 bp DNA fragment of F gene with IVS and polyA was cloned into multi clone site of vector SK with gB promotor of MDV.Finally,3 500 bp DNA fragment of F gene with gB promotor of MDV was cloned into vector SK containing US10,the nonessential fragment of MDV CVI 988.The research lays a foundation of obtaining recombinant MDV CVI 988 expressing NDV F gene.

关 键 词：新城疫病毒 F基因 马立克氏病病毒CVI988转移质粒载体 

分 类 号：S852.659[农业科学—基础兽医学] S852.659[农业科学—兽医学]