普通活性污泥富集好氧氨氧化菌试验  

Enrichment Cultivation of Ammonia Oxidizer from Normal Activated Sludge

作　　者：祖波[1] 张代钧[1] 卢培利[1] 张萍[1] 李小鹏[1] 

机构地区：[1]重庆大学资源与环境科学学院,重庆400030

出　　处：《重庆大学学报（自然科学版）》2005年第2期100-103,共4页Journal of Chongqing University

基　　金：国家自然科学基金(50378094);教育部优秀青年教师基金项目(教人司[2003]355号)

摘　　要：用普通活性污泥在SBR反应器中富集好氧氨氧化菌,反应器的运行周期为 1d,瞬间进水,曝气 21. 5h,沉淀 2h,排水 0. 5h。pH控制在 7. 8~8. 3,DO控制在 0. 8~1. 2mg/L,温度控制在 30±2℃。采用人工配水,初始人工配水中NH+4 -N的浓度为 50mg/L。待稳定后逐渐增加进水氨氮浓度至250mg/L,每次增加幅度 50mg/L。为定量判定富集过程中好氧氨氧化菌数目的增长情况,每隔 15d对SBR反应器中的污泥进行最大可能计数法(MPN法)测试和MLSS测试。富集培养 120d后,好氧氨氧化菌浓度提高 300倍。对比试验证实,pH和温度对好氧氨氧化菌的富集有明显影响。The mixed ammonia oxidizer culture was enriched from normal activated sludge in SBR reactor, in which the pH was controlled between 7.8 and 8.5,DO between 0.8 and 1.2 mg/L and temperature at 30±2 ℃. The operation cyclic of this reactor was 24 h, water following in instantly,aerating for 21.5 h, settling for 2 h, discharging for 0.5 h.The experimentation was carried out through synthetic wastewater,in which the initial NH^+_4-N was 50 mg/L and was increased up to 250 mg/L gradually by the step of 50 mg/L.The MPN and MLSS test were run every 15 days to characterize the enrichment of ammonia oxidizer.The concentration of the ammonia oxidizer culture increased by 300 times after the cultivation for 120 days. It is confirmed that pH and temperature has clear influence on the enrichment of ammonia oxidizer.

关 键 词：活性污泥 好氧氨氧化菌 富集培养 

分 类 号：X703[环境科学与工程—环境工程]