脑胶质瘤TGF-β_1和bFGF与增殖活性的相关性研究  

Study of correlation between expression of TGF-β_1 and bFGF and cellular proliferation in Human Glioma

作　　者：陈谦学[1] 黄乔春[1] 郑虎[1] 邵步云[1] 阮航[1] 王军民[1] 叶应湖[1] 

机构地区：[1]武汉大学人民医院神经外科,湖北武汉430060

出　　处：《中国现代医学杂志》2005年第2期227-229,共3页China Journal of Modern Medicine

摘　　要：目的探讨转化生长因子β1(TGF-β1)和碱性成纤维生长因子(bFGF)在人脑胶质瘤中的表达及其与胶质瘤增殖活性的关系。方法采用免疫组化方法,对临床经过石蜡包埋的35例胶质瘤和7例正常组织标本TGF-β1,bFGF和PCNA蛋白表达水平进行检测,并比较它们之间的相关性。结果TGF-β1,bFGF和PCNA在高级别胶质瘤(Ⅲ,Ⅳ级)中表达较多,在低级别胶质瘤(Ⅰ,Ⅱ级)中表达较少,在正常脑组织中几乎不表达;三者在高、低级别胶质瘤及正常脑组织中表达水平两两比较差异有显著性(P<0.05);且三者两两之间均呈显著正相关。结论TGF-β1和bFGF蛋白的阳性表达与胶质瘤细胞增殖活性和组织学分级密切相关;TGF-β1和bFGF的协同作用可能在胶质瘤细胞的增殖中具有重要意义。To investigate the expression of transforming growth factor -beta-1(TGF-β1) and basic fibroblast growth factor(bFGF) and its relationship with cellular proliferation in human glioma. The expression levels of TGF-β1, bFGF and PCNA in 35 cases of human glioma and 7 cases of normal brain tissue specimen were detected by immunohistochemical technics. Furthermore, the correlation among the expression of the TGF-β1, bFGF and PCNA was compared. In normal brain tissue, group Ⅰ,Ⅱand group Ⅲ,Ⅳ, the positive expression of TGF-β1 was 6.57±.72, 13.75 ±.14 and 36.07±0.13 respectively ,and bFGF was 9.29±.69, 23.85±.76 and 42.53±2.37 respectively ,and PCNA was 3.86±.07, 24.15±.12 and 57.07±7.26 respectively .The differences were all significant (P <0.05) in normal brain tissue, groupⅠ,Ⅱ and group Ⅲ,Ⅳ. TGF-β1, bFGF and PCNA were all positive correlation. [Conclusion] There is an intrinsic correlation between the expression of the TGF-β1, bFGF and cellular proliferation and histological grade of human brain gliomas. These findings suggest that the synergistic interaction of TGF-β1 and bFGF plays an important role during the cellular proliferation of glioma.

关 键 词：神经胶质瘤 转化生长因子Β1 碱性成纤维生长因子 增殖细胞核抗原 

分 类 号：R739.41[医药卫生—肿瘤]