乙酸镍诱导的人支气管上皮细胞系转化细胞基因组不稳定性分析  

Genomic Instability in Transformed 16HBE Cells Induced by Nickel Acetate

作　　者：陈传德[1] 吴中亮[2] 陈家堃[2] 

机构地区：[1]深圳市罗湖区疾病预防控制中心,广东深圳518020 [2]广州医学院化学致癌研究所,广东广州510182

出　　处：《癌变．畸变．突变》2005年第1期25-26,29,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(No.39170651)

摘　　要：背景与目的:检测乙酸镍对基因组不稳定性的影响,从而进一步探讨镍化合物致癌的分子机制。材料与方法:采用随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)技术来对经乙酸镍在诱导人支气管上皮细胞系(16HBE)的恶变细胞中的基因组不稳定性进行分析。结果:本实验所选用的7条随机引物均能扩增出清晰、明显的条带,条带数在1-6条之间。7条引物中第4、7两条引物扩增的片段在实验组和对照组之间无差异。其余五条引物均有差异,对于同一随机引物它们都具有特异的带型。结论:乙酸镍能诱发16HBE细胞基因组不稳定。BACKGROUND & AIM: To detect the genomic instability in the 16HBE cells induced by nickel acetate. MATERIAL AND METHODS: Analysing the genomic instability in the 16HBE cells induced by nickel acetate by random amplified polymorophic DNA(RAPD) .RESULTS:Compared with the negative control cells, 16HBE cells induced by nickel acetate have special PCR bands, indicating genomic instability. But the bands amplified by forth and seventh primes have no difference. CONCLUSION: Crystalline nickel sulfide could induce genomic instability in the 16HBE cells induced by nickel acetate.

关 键 词：人支气管上皮细胞系 细胞基因 诱导 基因组不稳定性 随机扩增多态性DNA 对照组 随机引物 RAPD 带型 乙酸 

分 类 号：R135[医药卫生—劳动卫生] R995[医药卫生—公共卫生与预防医学]