电针对束缚应激大鼠下丘脑神经元活化的调节作用  

The study on the regulation of electroacupuncture to the neuron activity in thalamus from restrain stress rat

作　　者：万顺伦 [1] 孙锦平 [2] 李召峰 [3] 

机构地区：[1]济南军区青岛第一疗养院,266071,青岛 [2]100853,北京,解放军总医院神经信息中心 [3]第二军医大学生理教研室硕士研究生

出　　处：《中国行为医学科学》2005年第2期162-164,共3页Chinese Journal of Behavioral Medical Science

摘　　要：目的 观察应激大鼠下丘脑神经元的活化程度,以及电针足三里穴对它的调节作用。方法将大鼠随机分为对照组、束缚应激组、束缚应激加针刺组,以标志神经元活化的c fos蛋白作为检测指标,用Western印迹杂交法观察三组大鼠下丘脑内c fos蛋白的变化。结果 束缚应激大鼠下丘脑内c fos蛋白表达水平明显高于对照组(P<0. 01)。束缚应激大鼠经针刺足三里穴后下丘脑内c fos蛋白与应激组相比明显回落,在针刺结束后的各个时间点(0h、1h、3h)下丘脑内c fos蛋白均比应激组有所下降,其差异均具有显著性意义(P<0. 05)。结论 束缚应激能够引起下丘脑c fos蛋白的表达增加,针刺足三里穴能够下调下丘脑c fos蛋白的表达水平,针刺可能在预防应激性疾病中具有重要作用。Objective To observe the effects of restrain stress on the neuron activity in the rat thalamus and the regulation of electroacupuncture to neuron activity induced by restrain stress.Methods Rats were randomly divided into three groups, including control group, restrain stress group and restrain stress plus electroacupuncture group. The Western blot was employed to observe the changes of c-fos expression marking neuron activity in the above three groups. Results Western blot analysis demonstrated that a higher level of c-fos protein was found in the thalamus from restrain stress group than that from control. The higher level of c-fos could last for 3 hours after stress relieved.The level of c-fos could be induced to decrease more or less when the Susanli acupoint of restrain rat was acupunctured. The effects of electroacupuncture of Susanli on c-fos in thalamus could still be kept from the time zero-hour to three-hour point after the electroacupuncture was stopped. Conclusion These results suggest that restrain stress can lead a higher level of c-fos in thalamus, which may be an important event in the response of HPA axis. The effect of electroacupuncture of Susanli on the c-fos is opposite to that of restrain stress. The electroacupuncture of Susanli may be a good way in preventing stress-related disease.

关 键 词：束缚应激 下丘脑 大鼠 脑内 S蛋白 c—fos 针刺 目的观 结论 重要作用 

分 类 号：R245[医药卫生—针灸推拿学]