脱色希瓦氏菌（Shewanella decolorationis）S12^T的脱色特性  

The Decolorizing Characterization of Shewanella decolorationis S12T

作　　者：许玫英[1] 钟小燕[1] 曹渭[1] 郭俊[1] 岑英华[1] 孙国萍[1] 

机构地区：[1]广东省微生物研究所

出　　处：《微生物学通报》2005年第1期5-9,共5页Microbiology China

基　　金：国家"863"高技术研究发展计划项目(No.2001AA214111);广东省自然科学基金团队项目资助(No.015017)

摘　　要：从印染废水活性污泥中分离到一株高效染料脱色菌,经鉴定该菌株为希瓦氏菌属的一个新种,命名为脱色希瓦氏菌(Shewanelladecolorationis)S12T。该菌株在偶氮染料浓度为50mg/L的培养基中培养4h后,染料去除率达到96%,对偶氮染料的最高脱色浓度达到2000mg/L。在浓度为500mg/L的偶氮染料平板上生长4d后,可观察到明显的脱色圈。全波长光谱扫描的结果表明希瓦氏菌S12T以生物降解的方式对偶氮染料进行脱色。希瓦氏菌S12T的脱色酶为组成型的胞内酶。High efficient dyes decolorizing bacterium, strain S12~T, was isolated from activated-sludge of textile-printing wastewater treatment plant. The strain was identified as a novel specie of the genus Shewanella, for which the name Shewanella decolorationis sp. nov. is proposed. It's decolorizing rate reached 96% in 4h, when 50mg/L of azo dye was used. The organism exhibited a remarkable color removal capability, even at azo dye's concentration of 2,000mg/L. A clear decolorizing zone around each colonies appeared after four days grown on LB plate containing 500mg/L azo dye. The changes of UV-visible spectra of azo dye solution indicate that the color removal was largely attributed to biodegradation. The decolorizing enzymes of strain S12~T were constitute type and not secreted to the culture medium.

关 键 词：脱色希瓦氏菌S12^T 偶氮染料 脱色 

分 类 号：X172[环境科学与工程—环境科学]