石油生物脱硫菌Agrobacterium tumefaciens UP-3  

Study on Immobilization of Petroleum Biodesulfurization Catalyst Agrobacterium tumefaciens UP-3

作　　者：侯影飞[1] 孔瑛[1] 杨金荣[1] 辛伟[1] 张建辉[1] 

机构地区：[1]石油大学华东化学化工学院,东营257061

出　　处：《微生物学通报》2005年第1期52-56,共5页Microbiology China

基　　金：中国石油天然气集团公司中青年创新基金资助项目(No.200109);石油大学(华东)博士生创新基金资助项目(No.B200406)

摘　　要：对能降解二苯并噻吩(DBT)的根癌土壤杆菌AgrobacteriumtumefaciensUP3菌株进行了固定化研究,以聚乙烯醇(PVA)和海藻酸钠(SA)混合物为包埋法固定化载体,固定化最佳操作条件为4℃交联,PVA和SA混合物总浓度7%,两者最佳浓度比为6,细胞浓度为0.05g/mL。当DBT加入量为2.7mmol/L时,UP-3的静息细胞最高脱硫率为13%,而固定化细胞的脱硫效率超过了60%;固定化细胞的最佳使用条件为降解5d,温度28℃～32℃。Immobilization of the bacterium Agrobacterium tumefaciens UP-3 was studied in this paper. The results showed that the immobilized cells with the mixture of polyvinyl alcohol (PVA) and sodium alginate (SA) as the immobilizing carrier had good biodesulfurization characteristics; The optimum operation immobilization conditions were 4℃, the total concentration of PVA and SA being 7%(wt), and the concentration of cells being 0.05 g/mL. When DBT addition was 2.7 mmol/L, the DBT degradation of immobilized cells was above 60% while that of resting cells is 13%. The optimum degradation time and temperature of immobilized cells were 5d and 28℃～32℃, respectively.

关 键 词：石油生物脱硫 二苯并噻吩 固定化细胞 根癌土壤杆菌UP-3 

分 类 号：Q939.97[生物学—微生物学]