rhIFN-α、rhIL-2对血管内皮细胞调控的实验研究  

Regulation of rhIFN-α and rhIL-2 on vascular endothelia l cell in vitro

作　　者：雷燕[1] 高倩[2] 林燕林[1] 陈可冀[1] 石太新[2] 

机构地区：[1]中国中医研究院西苑医院,北京100091 [2]河南省新乡医学院,河南新乡453003

出　　处：《中国病理生理杂志》2005年第2期234-237,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .30 0 70 95 6 ) ;国家重大基础研究"973"课题 (No .2 0 0 0 0 5 70 0 0 )

摘　　要：目的 :探讨干扰素 -α(rhIFN -α)和白细胞介素 - 2 (rhIL - 2 )对人脐静脉内皮细胞增殖、迁移、细胞周期及血管内皮生长因子 (VEGF)含量的影响。方法 :以离体培养的人脐静脉内皮细胞 (humanumbilicalveinendothe lialcells,HUVEC)为模型 ,采用MTT法测定细胞增殖 ,流式细胞术分析其细胞周期 ,琼脂糖刮除法检测其对内皮细胞迁移的影响 ;采用酶联免疫吸附实验 (enzyme -linkedimmunosorbentassay ,ELISA)检测上述细胞因子作用下培养的HUVEC血管内皮生长因子 (VEGF)在上清液中的含量。结果 :rhIFN -α组细胞增殖和迁移数分别为 0 .199± 0 . 0 0 9和75 .75 0± 2 3. 330 ,rhIL - 2组为 0 . 2 17± 0. 0 0 5和 4 9. 2 5 0± 8. 14 0 ,而合用组为 0 .183± 0 . 0 80和 4 0 . 5 0 0± 17. 2 30 ,与对照组(0 . 2 4 8± 0 . 0 0 5和 16 0 .5 0 0± 13 .2 2 0 )比较 ,有显著差异 (均P <0 . 0 1) ;细胞周期中S期细胞数目 ,rhIFN -α、rhIL - 2单用或合用组分别为 14 .18± 0 . 5 5、13 31± 0 . 78、10. 0 5± 0 . 4 3,而对照组为 15 . 99± 1. 0 2 (P <0 .0 5或P <0 . 0 1)。单独应用rhIFN -α或rhIL - 2可显著增加上清液中VEGF含量 (P <0 . 0 1) ,而合用组上清液中VEGF水平与对照组比较 ,差异无显著 (P >AIM: To study the effects of rhIFN-α and r hI L-2 on human umbilical vein endothelial cell (HUVEC) proliferation, migration, cell cycle and vascular endothelial growth factor (VEGF). METHODS: Cultured HUVECs were used as model to determine the cel l proliferation with MTT method. Cell cycle was analyzed by cytometry. The cell migration was investigated by agarose scraping method and vascular endothelial g rowth factor (VEGF) content in supernatant of cultured HUVEC was determined by e nzyme-linked immunosorbent assay (ELISA). RESULTS: The growth and migrating number of endothelial cells in rhIFN-α group were 0.199±0.009 and 75.750±23.330, in rhIL-2 group was 0 .217±0.005 and 49.250±8.140, and in combined group was 0.183±0.080 and 40.500±17.230, respectively. In comparison with control group (0.248±0.005 and 160.500±13.220), the effects showed more significant (all P<0.01). By treating the cells with rhIFN-α, rhIL-2 or in combination of both, the cell population in S phase was 14.18±0.55, 13.31±0.78 or 10.05±0.43, respect ively and control group was 15.99±1.02 (P<0.05 or P<0.01). VEGF con tent in supernatant of HUVEC culture in rhIFN-α or rhIL-2 group was higher than those in control group (P<0.01), but VEGF content between in combination g roup and in the control group was insignificant (P>0.05). CONCLUSIONS: rhIFN-α and rhIL-2 show inhibitory effect on vasc ular endothelial cell proliferation, migration and DNA synthesis. When used in c ombination, synergistic effect of rhIFN-α and rhIL-2 is observed, suggesting th at these cytokines play an important role in angiogenesis diseases.

关 键 词：干扰素Α 白细胞介素2 内皮细胞 细胞增殖 细胞周期 内皮细胞生长因子 

分 类 号：R363[医药卫生—病理学]