中华眼镜蛇毒F组份对血小板活化时蛋白酪氨酸磷酸化的影响  

Effect of fraction F of naja naja atra venom on protein tyrosine phosphorylation in platelet activation

作　　者：张梅[1] 余清声[1] 

机构地区：[1]广州医学院广州蛇毒研究所,广东广州510182

出　　处：《中国病理生理杂志》2005年第2期293-295,共3页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目 (No .980 4 6 3) ;卫生部科学基金资助项目 (No .98- 2 - 349)

摘　　要：目的 :观察中华眼镜蛇毒F组份抑制血小板聚集的胞内信号转导机制。方法 :实验分 6组 :(1)空白组 ;(2 )对照组 ;(3) - (6 )实验组加入浓度为 10 0、30、10、3mg/LF组份。用比浊法测定血小板聚集率。用蛋白质免疫印迹法 (Westernblotting)测定血小板内蛋白酪氨酸磷酸化的表达。结果 :F组份呈浓度依赖性抑制二磷酸腺苷 (ADP)引起的分子量为 76、6 6和 37 5kD蛋白酪氨酸磷酸化的表达 ,其中 30、10 0mg/L给药组中 ,3个分子量蛋白与对照组比较均有显著差异 ,P <0 . 0 5。F组份对ADP引起血小板聚集作用的抑制同降低 76、6 6和 37 5kD 3个分子量蛋白酪氨酸磷酸化的表达呈明显的正相关 (r=0 . 936 7,P <0 . 0 1)。结论 :中华眼镜蛇毒F组份通过抑制分子量为 76、6 6和37 5kD蛋白的酪氨酸磷酸化表达 ,对血小板胞内信号转导过程发生影响 ,可能是其抗栓机制之一。AIM: To observe the mechanism of intracellu la r signal transduction that fraction F of naja naja atra venom inhibits plate let aggregation. METHODS: Tests were divided into six groups: (1) blank group; (2 ) control group and (3)-(6) ADP plus fraction F group (doses of fraction F were 100, 30, 10, 3 mg/L, respectively). Protein tyrosine phosphorylation in platelet s was assayed by Western blotting and platelet aggregation was assayed by nephel omete r. RESULTS: Fraction F significantly inhibited molecular masses (MW ) 76, 66 and 37.5 kD protein tyrosine phosphorylation in platelet that induced by ADP in a dose-dependent manner, in which 30 and 100 mg/L dose group showed ob viously different effects when compared to control group (P<0.05). Inhibiti on of platelet aggregation was positive correlated with protein tyrosine phospho rylation in platelets (r=0.9367, P<0.01). CONCLUSIONS: Fraction F of naja naja atra venom affected int racellular signal transduction pathway in platelets by inhibiting MW 76, 66 and 37.5 kD protein tyrosine phosphorylation. The result suggests that this effect may be one of the anti-thrombus mechanisms of fraction F.

关 键 词：眼镜蛇毒液类 血小板聚集 印迹法 蛋白质 蛋白质酪氨酸磷酸化 信号转导 

分 类 号：R363[医药卫生—病理学]