Flt-4在不同级别脑星形细胞瘤中的表达意义  

Significance of expression of Flt-4 in different histopa thological grades of astrocytoma

作　　者：叶秀峰[1] 钟雪云[2] 米粲[1] 吴赤蓬[3] 

机构地区：[1]重庆医科大学病理教研室,重庆400016 [2]暨南大学医学院病理教研室,广东广州510632 [3]暨南大学医学院公共卫生教研室,广东广州510632

出　　处：《中国病理生理杂志》2005年第2期380-382,共3页Chinese Journal of Pathophysiology

基　　金：广东省科技厅研究团队项目课题 (No .0 15 0 12 ) ;广州市科技局科研重点立项课题 (No .2 0 0 1-E - 0 1- 2 ) ;国家科技部重大基础研究前期研究专项 (No .2 0 0 2ccc0 4 0 0 )

摘　　要：目的 :探讨不同组织病理分级的脑星形细胞瘤中血管内皮生长因子受体 3(Flt- 4又称VEGFR - 3)的表达意义。方法 :采用免疫组织化学方法 ,检测 5 0例不同级别脑星形细胞瘤患者手术切除标本中Flt - 4、血管内皮生长因子 (VEGF)的表达 ,并用抗FⅧ因子抗体标记瘤组织血管内皮细胞 ,计算肿瘤内微血管密度 (IMVD)。结果 :Flt- 4、VEGF总阳性表达率分别为 5 2 % (2 6 / 5 0 )、6 0 % (30 / 5 0 )。Flt- 4、VEGF均与脑星形细胞瘤病理分级呈显著正相关 (等级相关系数分别为 0 . 35 9、0 . 36 0 ,P <0 . 0 5 )。结论 :脑星形细胞瘤中有Flt- 4表达 ,主要表达于血管内皮细胞和部分肿瘤细胞 ,Flt- 4既可是内皮细胞自分泌产生 ,部分还可来自瘤细胞的旁分泌 ;脑星形细胞瘤中Flt- 4阳性表达的脉管是血管 ;Flt- 4的表达与星形细胞瘤的病理分级相关。AIM: To study the significance of expressio n of fms-like tyrosine kinase 4 (Flt-4) in different histopathological grades of a strocytoma. METHODS: The surgical specimens from 50 brain astrocytoma patien ts were stained immunohistochemically for examining Flt-4 and vascular endotheli al growth factor (VEGF) expression. Intratumoral microvessel density (IMVD) was calculated by labeling the endothelial cells of the blood vessels within the tum or. RESULTS: Flt-4, VEGF expression were closely correlated with his topathological grades of astrocytoma. Flt-4 and VEGF expression were found in 52 % (26/50), 60% (30/50) of tumors. A significant correlation was found between Fl t-4 and VEGF expression (P<0.01). CONCLUSIONS: Flt-4 is presented in human brain astrocytoma. Flt- 4 expression is mainly expressed in vascular endothelial cells and some tumor ce lls. Flt-4 may not only come from autocrine secretion of vascular endothelial ce lls, but also from paracrine secretion of tumor cells. The expression of Flt-4 i s significantly associated with histopathological grades of astrocytoma.

关 键 词：星形细胞瘤 FLT-4 血管形成 病理性 

分 类 号：R363[医药卫生—病理学]