HSV-tk与p53基因对涎腺多形性腺瘤细胞的杀伤作用  

The Effects of HSV-tk Suicide Gene and Wild-type p53 Gene on Pleomorphic Adenoma Cells of Salivary Gland

作　　者：王旭[1] 王洁[1] 董福生[2] 董玉英[2] 侯亚丽[1] 

机构地区：[1]河北医科大学口腔医学院病理室 [2]河北医科大学口腔医院口腔颌面外科,河北石家庄050017

出　　处：《华西口腔医学杂志》2005年第1期65-68,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30271422);国家留学人员科技活动择优资助项目(2002年);河北省自然科学基金资助项目(C2004000624)

摘　　要：目的　观察腺病毒介导的单纯疱疹病毒胸苷激酶(HSV_tk)基因、野生型p53基因(wt_p53)联合杀伤人涎腺 多形性腺瘤细胞的效果。方法　采用腺病毒介导的HSV_tk基因、wt_p53基因联合转染人涎腺多形性腺瘤细胞,逆 转录聚合酶链式反应(RT_PCR)检测转染后的基因表达;四唑盐比色(MTT)法测定tk与p53基因治疗后对肿瘤细 胞的杀伤作用及旁观者效应,并采用相差显微镜观察基因治疗后肿瘤细胞的形态学变化。结果　单独转染wt_p53 及HSV_tk GCV基因5d后,涎腺多形性腺瘤细胞的存活率分别为54%和38%;两者联合转染5d后,细胞的存活率 降至20%,两者差异有显著性(P<0.05)。结论　HSV_tk、wt_p53基因联合应用对涎腺多形性腺瘤的杀伤作用强于 此两基因单独应用的杀伤作用。Objective To study the therapeutic effects of combined gene therapy of wild type p53 (wt-p53) and herpes simplex virus thymidine kinase (HSV-tk) gene on pleomorphic adenoma cells of salivary gland.Methods Wild type p53 and HSV-tk gene were transfected into human pleomorphic adenoma cells of salivary gland by using recombinant adenovirus vector. The efficiency of transfection was checked and gene was expressed by RT-PCR methods. The cell inhibition after transfected was verified by light microscope and MTT.Results The proliferation of the pleomorphic adenoma cells transfected wt-p53 and HSV-tk gene was inhibited and the cell survival rate decreased to 54% and 38% respectively in 5 days. However, when wt-p53 gene combined with HSV-tk/GCV system, the killing effects was significantly stronger (P<0.05) and the cell survival rate decreased to 20%.Conclusion Combining p53 gene with HSV-tk/GCV system for gene therapy in pleomorphic adenoma cells of salivary gland is a valuable method.

关 键 词：腺瘤 涎腺 基因治疗 野生型P53 单纯疱疹病毒胸苷激酶基因 

分 类 号：R739.8[医药卫生—肿瘤]