重组人干细胞因子/血小板生成素融合蛋白的纯化及复性研究  

Purification and renaturation of recombinant human SCF-TPO fusion protein

作　　者：刘楠[1] 奚永志[1] 孙玉英[1] 郭斯启[1] 袁志宏[1] 崔建武[1] 习彩霞[1] 梁飞[1] 孔繁华[1] 

机构地区：[1]军事医学科学院附属医院及国家生物医学分析中心免疫学研究室

出　　处：《中国输血杂志》2005年第1期1-3,共3页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金资助课题(No39800056);国家博士后基金资助课题

摘　　要：目的　探讨重组人干细胞因子/血小板生成素(SCF TPO)融合蛋白纯化及复性的最佳方法。方法　裂解 法提取包涵体,在变性条件下利用金属螯和层析获得融合蛋白,经透析及谷胱甘肽氧化还原法对纯化的融合蛋白进 行复性。结果　获得具有初步生物学活性的SCF TPO融合蛋白,其纯度高达90%。结论　该纯化方法操作简捷, 特异性高,纯化效果好,获得率高。Objective To study the purification and renaturation of recombinant human SCF-TPO fusion protein. Methods After the inclusion bodies were isolated, the optimal conditions for denaturation and renaturation were studied. The denatured recombinant human SCF-TPO fusion protein was purified by metal chelating chromatography and renatured by dialysis in GSH/GSSH. Results The purified SCF-TPO fusion protein manifested primary biological activity with a purity of up to 90%. Conclusion The purification procedures employed in this study is simple and reliable, with a high specificity and good purification rate.

关 键 词：人干细胞因子/血小板生成素融合蛋白 包涵体 纯化 复性 

分 类 号：R392.11[医药卫生—免疫学]