改良的大鼠脑局部缺血模型  

Modified rat model of middle cerebral artery occlusion

作　　者：徐立新[1] 袁芳[1] 

机构地区：[1]北京市神经外科研究所,北京市100050

出　　处：《中国康复理论与实践》2005年第1期23-24,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市科技新星计划项目 (95381 30 0 0 ) ;北京市自然科学基金项目 (70 4 30 65)。

摘　　要：目的介绍一种快速、稳定的大鼠脑局部缺血模型。方法 3 2只Wistar雄性大鼠 ,按照Longa的线栓法制作局部脑缺血模型 ,但在放入栓塞线时不结扎翼腭动脉 (PPA) ,通过局部脑血流监测、动物行为学评分、脑含水量测定、红四氮唑 (TTC)染色和病理学检查等方法评价该模型的可靠性。结果动物麻醉后一般只需 15min左右即可完成栓线手术 ,术后大鼠大脑中动脉区域血流量下降 ,神经功能损伤明显 ,脑含水量增高 ,TTC染色脑梗死区苍白 ,病理学检查显示缺血灶星形胶质细胞肿胀 ,神经元固缩坏死。结论不结扎PPA的大鼠脑局部缺血模型 ,缺血效果明确可靠 ,手术时间短 ,是理想的研究脑缺血的实验模型。ObjectiveTo introduce a rapid and stable rat model of middle cerebral artery occlusion ( MCAO ). Methods32 Wistar male rats were used to induce focal cerebral ischemia according to Longa's method. The main difference from Longa's was to introduce a nylon suture into the cervical internal carotid artery without ligation the pterygopalatine artery (PPA). Regional cerebral blood flow ( rCBF ), neurological deficit, brain water content, red tetrzolium (TTC) stain and pathology were evaluated in all rats.ResultsThe operation proceeded about 15 min after anaesthesia. Rats after operation presented severe neurological deficits companied with rCBF decreases. There were significant increases of brain water contents in ischemic brain regions. TTC staining showed infarct areas and histological examinations revealed remarkable astrocyte swelling and neuronal necrosis. ConclusionInducing rat MCAO without ligation of PPA could produce reliable ischemic changes, with the advantage of shortening operation time. [

关 键 词：脑缺血 动物模型 翼腭动脉 大脑中动脉 大鼠 

分 类 号：R743.32[医药卫生—神经病学与精神病学]