养寿丹对Alzheimer病模型大鼠学习记忆的影响研究  

Experiment Research On The Learning And Memory Capacity Of Alzheimer Disease Rat After Using Yang Shoudan(YSD)

作　　者：王玲[1] 黄兆胜[1] 刘明平[1] 熊平[1] 孙维广 

机构地区：[1]广州中医药大学中药学院,广东广州510405 [2]广州奇星制药厂,广东广州510411

出　　处：《中医药学刊》2005年第1期136-138,141,共4页Study Journal of Traditional Chinese Medicine

摘　　要：养寿丹是出自《御药院方》的传统名方 ,主要由巴戟天、枸杞子、肉苁蓉、何首乌、当归、生白术、茯苓、远志、石菖蒲等中药组成 ,具有补肾益精、化痰活血、醒脑开窍的作用 ,针对AD患者肾精不足、气血亏虚、夹痰夹瘀的病机来治疗。目的 :研究传统名方“养寿丹”抗痴防呆的作用机理。方法 :对用D 半乳糖致亚急性衰老 ,合并Meynert核损毁所致Alzheimer病模型大鼠进行避暗穿梭实验和Morris水迷宫实验 ,测试其学习记忆能力。结果 :在避暗穿梭实验中 ,养寿丹能延长AD模型大鼠学习记忆潜伏期 ,减少错误次数 ;在Morris水迷宫定位航行实验中 ,养寿丹能明显缩短AD模型大鼠逃避潜伏期 ,在空间探索实验中 ,养寿丹能明显增加该大鼠在各象限跨越相应平台次数 ;这些作用以养寿丹高剂量组明显 ,低剂量组次之 ,它们的效果均较对照药石杉碱甲组稍好。结论 :养寿丹有改善AD模型大鼠学习记忆的能力 ,而且该作用比石杉碱甲还好。Objective :To research the mechanism of Yang Shoudan(YSD) on the prevention and treatment of AD. Methods :Established the model rat of Alzheimer's Disease(AD)induced by intraperitoneral injection of D galactose(50mg/d,for 40 days)and combined with ibotenic acid injection into bilateral nucleus basalis of Meynert (for two sides,5μg/per side,for one time)at first,and then observed the learning and probe capacity of rat by experiments on avoiding dark going through crooked road and Morris water maze test. Results :YSD can extend the latent period of learning and memory of AD rat and decrease the wrong frequency in avoiding dark going through crooked road experiment,and shorten the escape latent period of AD rat and increased the times of crossing the platform of its quadrant in Morris water maze test.This effect was much better in high dose of YSD than that in low dose and Huperzine A. Conclusion :YSD can improve the learning and memory and spatial probe capacity of AD rat,and these effects were better than that in Huperzine A.

关 键 词：养寿丹 ALZHEIMER病 学习记忆能力 避暗穿梭实验 Morris水迷宫实验 

分 类 号：R-05[医药卫生]