β_2-肾上腺素能受体基因+46位多态性与新疆哈萨克族人超重肥胖的关系  

The Association Study between β_2-Adrenergic Receptor +46 Variant and Body Mass Index (BMI) in Kazak of Xinjiang

作　　者：骆秦[1] 李南方[1] 周玲[1] 李红建[1] 李涛[1] 

机构地区：[1]新疆维吾尔自治区高血压研究所

出　　处：《新疆医科大学学报》2005年第1期8-11,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(30260038;30240045)

摘　　要：目的:通过观察β2-肾上腺素能受体基因(β 2-adrenergic receptor, β 2-AR、ADRB2)+46A→G (Arg16/Gly)多态性与体重指数(BMI)的关系,研究其与哈萨克族人超重肥胖的关系.方法:采集120例35～65岁哈萨克族人的遗传标本,按WHO超重和肥胖划分标准以BMI<25 kg/m 2、25～29.9 kg/m 2、≥30 kg/m 2 将人群划分为正常、超重及肥胖3组,采用allele-specific PCR方法检测和分析该人群β 2-AR基因+46A→G多态性,观察各基因型和等位基因频率在不同BMI水平的分布及其与超重肥胖的关系. 结果:该人群β 2-AR+46位基因型频率为AA 0.39,AG 0.37,GG 0.24;A等位基因频率为0.58,G等位基因频率为0.42;3组间相应AA、AG、GG及A、G基因型和等位基因频率分别为0.37、0.39、0.24、0.56 、0.44;0.47、0.33、0.20、0.63、0.37和0.29、0.38、0.33、0.48、0.52,3组基因型及等位基因频率差异均无统计学意义(P＞0.05). 结论:哈萨克族人群β 2-AR基因存在+46 A→G (Arg16/Gly)多态性,但该位点多态性与哈萨克族人群超重肥胖无关.Objective: To investigate the association of β-2-Adrenergic Receptor +46 Variant with overweight and obesity by BMI in kazak of Xinjiang. Methods: The study recuited 120 kazak aged from 35 to 65, who were devided into three groups according to WHO measures of overweight and obesity (BMI <25 kg/m+2, 25～29.9 kg/m+2, ≥30 kg/m+2). Allele-specific ploymerase chain reaction (PCR) procedures were developed to genetype the Arg16/Gly variant of β-2-AR gene. Results: The frequencies of genotypes AA, AG, GG and alleles A,G of β-2-AR +46 variant in total population were 0.37, 0.39, 0.24 and 0.56, 0.44; in group of BMI <25 kg/m+2 were 0.39, 0.37, 0.24 and 0.56, 0.44; in group of BMI 25~29.9 kg/m+2 were 0.47、0.33、0.20 and 0.58, 0.42; and in group of BMI ≥30 kg/m+2 were 0.39, 0.38, 0.33 and 0.48, 0.52, respectively. There were no significant difference in frequencies of both genotypes and alleles between the three groups of BMI in this population (P>0.05). Conclusions: These data show that +46A→G (Arg16/Gly) variant of β-2-AR gene was found in kazak, but may not correlate to BMI, overweight and obesity in this population.

关 键 词：β2-肾上腺素受体(β2-AR) 基因多态性 体重指数 肥胖 哈萨克族 

分 类 号：R151.1[医药卫生—营养与食品卫生学]