阿魏酸转化反应速率常数的毛细管电泳测定方法研究  

Determination of the Conversion Rate Constants of Ferulic Acid by Capillary Zone Electrophoresis

作　　者：付绍平[1] 李秀玲[1] 张峰[1] 徐青[1] 肖红斌[1] 梁鑫淼[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116012

出　　处：《分析测试学报》2005年第1期8-11,共4页Journal of Instrumental Analysis

基　　金：国家973计划"中药方剂关键科学问题基础研究"项目资助 (G1999054406)

摘　　要：初步确定了阿魏酸自然转化产物的结构 ,并用毛细管电泳测定了转化反应速率常数。在常温下 ,用毛细管区带电泳法直接测定阿魏酸浓度随时间变化的规律 ,并根据HPLC -MS -MS得到的质谱图及紫外光谱图初步确定了转化产物。在23℃ ,阿魏酸转化反应正向和逆向反应速率常数分别为5.194×10 -6s -1、5.360×10 -6s -1。方法简单、灵敏。Amethod based on capillary zone electrophoresis was developed for the determination of the converˉsion rate constants of ferulic acid.The temporal variation of trans-ferulic acid concentration was measured by capillary zone electrophoresis,and the structure of its conversion product—cis-ferulic acid was preliminarily confirmed with the aid of HPLC-MS-MS mass spectra,and ultraviolet spectra as well.The forward and backward reaction rate constants of ferulic acid conversion at23℃were found to be5.194×10 -6 s -1 and5.360×10 -6 s -1 ,respectively.The method is simple,sensitive,and fast.

关 键 词：毛细管区带电泳 阿魏酸 反应速率常数 

分 类 号：O657.8[理学—分析化学] O643.11[理学—化学]