人和常用实验动物牙龈结合上皮的形态结构比较研究  

Comparative study on the histomorphology of the JE of human and several laboratory animals

作　　者：姜茜[1] 李德懿[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学院口腔内科,上海200011

出　　处：《上海口腔医学》2004年第6期539-543,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30471886)

摘　　要：目的:研究人和常用实验动物牙龈结合上皮(Junctionalepithelium,JE)的形态结构,筛选适合实验用的动物、部位和方法。方法:分别选取6例人、3头猪、4条犬、6只家兔、6只大鼠下颌磨牙及牙龈颊舌向的黏膜组织石蜡切片,HE染色结合图像分析,研究JE的形态结构。结果:人、猪、犬、家兔和大鼠JE宽度分别为(1.019±0.133)mm、(0.862±0.073)mm、(1.111±0.104)mm、(0.404±0.010)mm和(0.285±0.032)mm,均占龈沟上皮(Gingivalsulcularepithe鄄lium,GSE)与JE宽度和的60%左右。猪、狗JE形态结构与人相似,细长呈条索状,形态规则,向冠方增厚,与GSE连续,两者可从细胞形态和染色上区别;家兔、大鼠与人差别较大,JE位于GSE牙面侧,形态不规则,向根方增厚,冠方与GSE分离,GSE角化明显。结论:猪、狗的JE形态结构、牙颌发育与人十分接近,可作为JE研究的首选实验动物;掌握人和常用动物的JE解剖、生理特点及取材要点,筛选合适的研究对象,可为进一步研究JE生物学特性提供有用的参考资料和方法。PURPOSE: To investigate the histomorphology of the junctional epithelium (JE) of human and several animals, and determine the suitable laboratory animals, position and methods for JE study. METHODS: Paraffin-embedded bucco-lingual sections of mandible molars with gingival tissue from 6 men, 3 pigs, 4 dogs, 6 rabbits and 6 rats were stained by HE and analyzed by image analysis system. RESULTS: The width of JE of human, pig, dog, rabbit and rat was(1.019±0.133)mm, (0.862±0.073)mm, (1.111±0.104)mm, (0.404±0.010)mm and (0.285±0.032)mm,respectively, and the ratio of the width of JE to the gingival sulcular epithelium(GSE) and JE was about 60%. The histology and morphology of JE of pig and dog were similar to that of human, the epithelium was long, thin, regular and connected with GSE. The demarcation between JE and GSE was judged by the differences in the cytology and staining. The JE of rabbit and rat was irregular and quite different from that of human. It was located on the surface of GSE, turned wide towards its bottom and the top of it was separated from GSE which was remarkably keratinized. CONCLUSIONS: The most suitable animals for JE study are pig and dog because of their similarity to human on the morphology and development of JE. The mastery of the anatomical and physiological characteristics of JE of human and several laboratory animals and determination of the suitable objects would provide useful data and methods for further study of the biological and molecular characters of JE cells.

关 键 词：结合上皮 形态结构 图像分析 

分 类 号：R780.2[医药卫生—口腔医学]