尿多酸肽在人胶质瘤中的抗瘤作用  

Anti-tumor Effect of Uroacitide on Human Glioma Cell Line

作　　者：王红艳[1] 钟雪云[1] 刘致中[1] 詹美意[1] 

机构地区：[1]暨南大学医学院病理教研室,广州市510632

出　　处：《中国肿瘤临床》2005年第3期139-143,共5页Chinese Journal of Clinical Oncology

基　　金：国家科技部重大基础研究前期研究专项(973)(编号:2002ccc0400);广东省科技厅研究团队项目(编号:015012); 广州市科技局科研重点立项基金资助(编号:2001-E-01-2)

摘　　要：目的:研究人尿萃取物尿多酸肽(CDA-Ⅱ)对人胶质瘤细胞SWO-38增殖及分化的影响。方法:体外实验采用MTT法、集落形成试验检测CDA-Ⅱ对SWO-38细胞存活与集落形成的影响,体内实验利用裸鼠移植瘤试验观察CDA-Ⅱ抗胶质瘤生长的作用。HE染色观察CDA-Ⅱ作用后SWO-38的形态学改变。结果:SWO-38经1～5mg/mLCDA-Ⅱ处理3天后,细胞增殖抑制率为39.49%±5.27%～65.05%±5.89%,CDA-Ⅱ的IC50为2.52mg/mL;0.3～2.1mg/mLCDA-Ⅱ处理SWO-3810天后,集落形成抑制率为23.45%±0.62%～96.22%±1.01%,IC50为1.03mg/mL。这种抑制效应呈剂量依赖性。经腹腔注射给药28天后,见高低剂量CDA-Ⅱ均可显著抑制裸鼠体内SWO-38增殖,抑瘤率为79.94%、47.77%(P<0.05,n=10)。CDA-Ⅱ诱导SWO-38出现细胞分化的形态学改变。结论:CDA-Ⅱ对人胶质瘤细胞SWO-38有较好的抗瘤作用,是很有潜力的天然抗胶质瘤药物。Objective: To exam the proliferation and differentiation effect on human glioma cell line SWO-38 by Uroacitide (CDA-Ⅱ), which is a healthy human urine extraction. Methods: The effect of CDA-Ⅱon cellular survival and colony formation was determined by MTT assay and colony formation assay. Then the anti-tumor effect of CDA-Ⅱwas examined by transplanting SWO-38 in nude mice in vivo. Finally, the change of cell morphology was observed by HE staining and light microscopy. Results: After SWO-38 cells were treated with 1~5 mg/mL CDA-Ⅱ for 3 days, the inhibition rate of cell growth was 39.49%±5.27% to 65.05%±5.89%, the IC50 of CDA-Ⅱ was 2.52 mg/mL, and 10 days after the treatment with CDA-Ⅱ 0.3~2.1mg/mL, the inhibition rate of colony formation was 23.45%±0.62%~96.22%±1.01% and the IC50 was 1.03 mg/mL. Furthermore, the inhibitory effect was in a dose-dependent manner. CDA-Ⅱat dosage of 500 mg/kg/d and 2000 mg/kg/d with i.p. administration for 4 weeks could significantly suppress the growth of human glioma cells SWO-38 in nude mice, the inhibition rates were 47.77% and 79.94%, respectively (P<0.05, n=10=. HE staining and light microscopy revealed that CDA-Ⅱ could induce differentiation of human glioma cells SWO-38. Conclusion: CDA- Ⅱdemonstrates a significant anti-tumor effect on human glioma and is a potential and natural anti-glioma drug.

关 键 词：CDA—Ⅱ 胶质瘤 抗瘤作用 增殖诱导分化 

分 类 号：R739.4[医药卫生—肿瘤]