血管性痴呆患者皮肤α-微管蛋白免疫细胞化学与胶体金免疫的电镜观察  

Microscopy observation of immunocytochemistry and immunoelectron of colloidal on alpha-tubulin in skin of patients with vascular dementia

作　　者：查彩慧[1] 郭国庆[2] 肖计划[3] 

机构地区：[1]广州市儿童医院儿保科,广东省广州市510120 [2]暨南大学医学院解剖学教研室,广东省广州市510630 [3]暨南大学医学院神经病学教研室,广东省广州市510630

出　　处：《中国临床康复》2005年第5期62-63,共2页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(39570267)~~

摘　　要：目的:观察血管性痴呆(vasculardementia,VD)患者皮肤基底细胞α-微管蛋白的变化,从细胞的超微结构变化探讨其皮肤基底细胞α-微管蛋白与VD的关系。方法:实验于2001-03/12在广州东山区福利院和暨南大学医学院进行。取广州东山区福利院志愿参加实验的正常老人和VD患者各5例背部皮肤,用免疫细胞化学和胶体金免疫电镜技术观察,并进行定量分析。结果:免疫细胞化学染色显示,对照组皮肤免疫阳性染色主要位于基底细胞的胞质(吸光度为0.814±0.152;体积积分吸光度为96.020±14.755);VD组阳性产物主要位于基底细胞层(吸光度为0.398±0.081;体积积分吸光度为41.800±7.823),与对照组比较差异有显著性意义(t=8.368,-54.220,P<0.05)。胶体金标记结果显示对照组基底细胞可见较丰富的微管,标记的胶体金多散在于胞质犤(15.50±3.41)个/细胞)犦。VD组基底细胞内微管较对照组增多,基底细胞标记的胶体金颗粒较对照组明显减少犤(11.38±3.27)个/细胞,t=4.120,P<0.05犦。结论:VD患者皮肤组织细胞骨架结构亦发生变化,提示痴呆可能是一种系统性的退行性疾病。了解皮肤组织的变化,可能有助于痴呆的非神经系统生物学诊断指标的建立。AIM:To observe the changes of α tubulin in skin from vascular dementia(VD) patients and investigate the relationship of α tubulin and vascular dementia from the changes of cellular ultrastructure. METHODS:The experiment was finished in welfare institute of Dongshan and Medical College of Jinan University, between March and December 2001.The skins of back from normal elders(5 cases) and vascular dementia patients(5 cases) from welfare institute of Dongshan were investigated by immunocytochemistry and immunoelectron microscopy of colloidal,and the results were quantitatively analyzed. RESULTS:The results in immunocytochemistry showed that the immunopositve staining were located in plasma of basal cells in control group(absorbance was 0.814±0.152 and volum integral absorbance was 96.020±14.755).The similar images were also caught in VD patients(absorbance was 0.398±0.081; volum integral absorbance was 41.800±7.823).There was significance difference(t=8.368,-54.220;P < 0.05). The results of colloidal displayed that the plasma of basal cells had more microtubules, and the number of colloidal golds was scattered in cytoplasm[(15.50±3.41) particles per cell].In basal cells of AD patients there were more microtubes than those in control group, however, the particles of colloidal decreased[(11.38±3.27) particles per cell] significantly than that in control group(t=4.120,P< 0.05). CONCLUSION:The cellular skeletons in skin constitution of vascular dementia patients are easy to change and their changes may be one of phenomena in regression of neurons.Having known these changes in skin may be contributed to set up and unneurobiologic diadynamic criteria.

关 键 词：痴呆 血管性 微管蛋白 皮肤 免疫化学 显微镜检查 免疫电子 

分 类 号：R749.1[医药卫生—神经病学与精神病学]