“免疫-脑通讯”通路中延髓内脏带至下丘脑室旁核儿茶酚胺能作用的实验研究(英文)  

Experimental study on the catecholaminergic effects of paraventricular nucleus projected from medullary visceral zone in “immune-to-brain communication” access

作　　者：杨志军[1] 徐如祥[1] 饶志仁[2] 魏玲[3] 陈良为[2] 段丽[2] 姜晓丹[1] 蔡颖谦[1] 杜谋选[1] 

机构地区：[1]南方医科大学珠江医院神经外科,广东省广州市510282 [2]解放军第四军医大学神经科学研究所,陕西省西安市710032 [3]解放军第四军医大学唐都医院呼吸科,陕西省西安市710038

出　　处：《中国临床康复》2005年第5期212-214,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:脂多糖(lipopolysaccharide,LPS)作为一种多克隆免疫激发剂刺激免疫细胞,来模拟免疫激发状态,观察延髓内脏带(medullaryvisceralzone,MVZ)投射至下丘脑室旁核(paraventricularnucleusofhypothalamus,PVN)的儿茶酚胺神经元是否对LPS刺激起反应,为脑功能保护研究提供理论依据。目的:观察MVZ投射至PVN的儿茶酚胺神经元是否对脂多糖刺激起反应,探讨MVZ至PVN的儿茶酚胺能通路在“免疫-脑通讯”中的作用。设计:以实验动物为研究对象,随机对照的研究。单位:一所军医大学的神经外科和神经科学研究所。材料:实验在南方医科大学珠江医院神经外科和解放军解放军第四军医大学全军神经科学研究所完成。10只健康清洁级成年SD大鼠由第四军医大学实验动物中心提供。方法:将WGA-HRP注入大鼠一侧PVN内,存活48h后经腹腔注射免疫刺激剂LPS诱发免疫反应,应用WGA-HRP逆行追踪与抗Fos和抗酪氨酸羟化酶(TH)抗体双重免疫组织化学相结合三重标记方法进行染色。主要观察指标:观察MVZ中WGA-HRP逆标细胞、Fos蛋白和儿茶酚胺能神经元(以TH为标记物)的分布及表达状况。结果:在MVZ内发现7种阳性神经元,即HRP,Fos和TH单标细胞,Fos/HRP,Fos/TH和HRP/TH双标细胞,Fos/HRP/TH三标细胞。BACKGROUND:Lipopolysaccharide(LPS),as a polyclonal immune exciter,can simulate immune excitation status,which is useful in the observation of whether the catecholaminergic neurons in paraventricular nucleus of hypothalamus(PVN) projected from medullary visceral zone(MVZ) react towards LPS stimulation that is to provide a theoretical gist for the researches on the protection of brain function. OBJECTIVE:To observe whether PVN catecholaminergic neurons projected from MVZ react towards LPS stimulation for the exploration of the impacts of MVZ PVN catecholaminergic access in “immune to brain communication'. DESIGN:A randomized controlled study using experimental animals as subjects. SETTING:An Institute of Neurosurgery and Neurology of one Military University of Chinese PLA. MATERIALS:The study was conducted in the Department of Neurosurgery of Zhujiang Hospital affiliated to Southern Medical University and the Institute of Neurology of the Fourth Military Medical University of Chinese PLA from January to December in 2002.Ten healthy adult SD rats in cleanness grade were obtained from the experimental animal center of the Fourth Military Medical University of Chinese PLA. METHODS:WGA HRP was injected into PVN of one side of the rat,and the immune exciter LPS was injected into the abdominal cavity after 48 hours of survival to induce immune response. Samples were stained by triple labels of WGA HRP method and double immunohistochemical staining of anti Fos and anti TH antibodies. MAIN OUTCOME MEASURES:To observe the distributions and expressions of WGA HRP labeled cells,Fos protein,and catecholaminergic neuron(labeled by TH) in MVZ. RESULTS:Seven immune reactive(IR) positive neurons were found in MVZ,i.e.,HRP,Fos or TH single labeled cells,Fos/HRP,Fos/TH or HRP/TH double labeled cells,and Fos/HRP/TH triple labeled cells.Fos/HRP double labeled neurons and Fos/HRP/TH triple labeled neurons accounted for 12.5%and 39.6%of HRP labeled cells respectively. CONCLUSION:MVZ reacts to LPS immune stimulation, w

关 键 词：延髓 神经免疫调节 下丘脑室旁核 免疫组织化学 大鼠 

分 类 号：R74[医药卫生—神经病学与精神病学]