单胺类递质对脑出血大鼠心脏损伤的应变效应(英文)  

Dependent effects of monoamine transmitters on the cardiac injury in cerebral hemorrhage rats

作　　者：许志强[1] 蒋晓江[1] 王景周[1] 陈曼娥[1] 

机构地区：[1]解放军第三军医大学大坪医院野战外科研究所神经内科三病区,重庆市400042

出　　处：《中国临床康复》2005年第5期224-226,共3页Chinese Journal of Clinical Rehabilitation

基　　金：重庆市卫生局重点资助项目(2001-2003)~~

摘　　要：背景:急性脑损害条件下,心脏病变发生率高,影响其预后。血浆单胺类递质的大量增加,可能是脑卒中后心脏产生应变效应的主要原因之一。目的:研究单胺类递质在大鼠脑出血心脏损伤中的作用,探讨脑心综合征发生、发展的机制。设计:以实验动物为研究对象,随机对照实验研究。单位:一所军医大学医院的野战外科研究所神经内科病房。材料:实验在大坪医院神经内科实验室完成。动物选用健康Wistar大鼠70只(雌雄不拘,体质量250～300g),动物由第三军医大学大坪医院动物中心提供。干预:大鼠右侧尾状核注射含胶原酶(1u/μL)和肝素(7u/μL)的生理盐水1μL以建立脑出血模型;对照组改注射生理盐水1μL。主要观察指标:采用高效液相-电化学法测定血浆去甲肾上腺素(noradrenaline,NE)含量,采用酶反应速率法测定血清肌酸磷酸激酶同工酶MB型(creatinekinase-MB,CK-MB)的变化。结果:大鼠脑内血肿开始形成的6h血浆去甲肾上腺素(noradrenaline,NE)、血清肌酸磷酸肌酶同工酶MB型(creatinekinase-MB,CK-MB)均显著升高犤分别为(2.782±0.244)μg/L和(3.504±0.742)μkat/L犦,并以脑出血24h血肿高峰期时最为显著犤分别为(3.517±0.200)μg/L,(5.474±1.540)μkat/L犦,随后逐渐下降,72h血浆NE降至术前水平犤(1.663±0.094)μg/L犦。BACKGROUND:Incidence of cardiac pathological changes is high under acute cerebral damage, which affects its prognosis.The massive increase of plasma monoamine transmitters might be one of the key reasons of the dependent effects of the heart after cerebral apoplexy. OBJECTIVE:To investigate the effects of monoamine transmitters on the cardiac injury in cerebral hemorrhage in rats for the exploration of the mechanism for the generation and development of cerebral cardiac syndrome(CCS). DESIGN:A prospective, randomized and controlled study using experimental animals as subjects. MATERIALS:Study was conducted in the Laboratory of Neurology of Daping Hospital.Seventy healthy Wistar rats in either gender with a body mass between 250 g and 300 g were obtained from the animal center of Daping Hospital affiliated to Third Military Medical University of Chinese PLA. INTERVENTIONS:1 μL of normal saline containing collagenase(1 μ/μL) and heparin(7 μ/μL) was injected into the right caudate nucleus of the rats to establish cerebral hemorrhage animal model, while 1 μL of normal saline was injected into the rats of control group. MAIN OUTCOME MEASURES:Plasma noradrenaline(NE) content was analyzed by high potent electrochemical method,changes of serous creatine kinase MB(CK MB) were analyzed by enzyme reaction rate method. RESULTS:Both plasma NE and serous CK MB significantly elevated at 6 hours since the formation of hematoma in the brain of the rat, which were(2.782±0.244) μg/L and(3.504±0.742) μkat/L respectively.Plasma NE and serous CK MB most significantly increased at 24 hours,the peak of hematoma after cerebral hemorrhage, which were(3.517±0.200) μg/L and(5.474±1.540) μkat/L respectively.NE and CK MB gradually decreased afterwards,and plasma NE returned to preoperative level at 72 hours [(1.663±0.094) μg/L]while serous CK MB still higher than preoperative level [(2.713±0.526) μkat/L]. CONCLUSION:Peripheral NE content elevation might participate the generation and development process of CCS

关 键 词：脑血管意外/病理生理学 心血管疾病/病理生理学 综合征 去甲肾上腺素 肌酸激酶 

分 类 号：R743[医药卫生—神经病学与精神病学]