大鼠视网膜和视神经中睫状神经营养因子mRNA的表达定位  

Expression and localization of ciliary neurotrophic factor mRNA in retina and optic nerve in rats

作　　者：张巍[1] 叶剑[1] 陈春林[1] 邹倩[1] 许建涛[1] 

机构地区：[1]解放军第三军医大学大坪医院野战外科研究所眼科,重庆市400042

出　　处：《中国临床康复》2005年第2期180-181,i008,共3页Chinese Journal of Clinical Rehabilitation

基　　金：重庆市科委应用基础研究资助项目(7437)~~

摘　　要：目的:探讨睫状神经营养因子ciliaryneurotrophicfactor,CNTF)保护视(网膜和视神经损伤神经元的作用,与CNTFmRNA在成年大鼠正常视神经和视网膜表达定位的相关性。方法:取8只正常成年SD大鼠的眼球和视神经,行冷冻切片。用地高辛标记的寡核苷酸探针对组织切片进行原位杂交,检测CNTFmRNA在视网膜和视神经的表达定位。结果:原位杂交结果显示,在正常视网膜组织,CNTFmRNA阳性表达信号位于内核层。在正常视神经中,CNTFmRNA阳性细胞沿其长轴呈串珠状排列。结论:正常成年大鼠视网膜组织,CNTFmRNA阳性表达位于内核层;视神经中,CNTFmRNA广泛表达。这种分布特点可能与CNTF能够促进损伤的视网膜神经节细胞的存活和轴突再生有关。AIM:To investigate the effect of ciliary neurotrophic factor(CNTF) in protecti ng neuron induced by retina and optic nerve injury, and the correlation of the e xpression and localization of CNTF mRNA in normal retina and optic nerve in adul t rats. METHODS:Frozen sections of eyeball and optic nerve in eight SD rats were made. In situ hybridization was conducted on the tissue sections by oligonucleotide pr obe marked by cardiox.The expressions and localization of CNTF mRNA were detecte d in retina and optic nerve. RESULTS:The result of in situ hybridization showed that the positive expressio n of CNTF mRNA in the normal retina tissue was in inner nuclear layer.The positi ve cells of CNTF mRNA in the normal optic nerve were in a beaded arrangement alo ng the long axis. CONCLUSION:The positive expression of CNTF mRNA in the normal retina tissue of adult rats is in inner nuclear layer,and the CNTF mRNA has a comprehensive expr ession in the optic nerve.The characteristics of distribution may have the relat ion with the fact that CNTF can promote the survival and axonal regeneration of injured retina ganglion cells.

关 键 词：睫状神经营养因子 视神经 视网膜 原位杂交 基因表达 

分 类 号：R774.1[医药卫生—眼科]