不同牙周状态下龈下菌斑中人类巨细胞病毒的检测  

Detection of human cytomegalovirus in chronic periodontitis patients

作　　者：李颖[1] 章锦才[2] 张蕴惠[3] 

机构地区：[1]广州中山大学光华口腔医学院牙周黏膜科,510055 [2]广东省口腔医院 [3]四川大学华西口腔医学院

出　　处：《实用口腔医学杂志》2005年第1期54-57,共4页Journal of Practical Stomatology

摘　　要：目的 :研究人类巨细胞病毒 (HCMV)与慢性牙周炎及其活动性的相关性。方法 :采集 62例慢性牙周炎患者 (男性 2 7例 ,女性 3 5例 )的牙周炎活动部位 ,牙周炎静止部位 ,以及轻度龈炎部位的龈下菌斑 ,使用SeekVi ralDNAkit试剂盒提取DNA ,采用巢式PCR法检测HCMV ,比较同一患者不同牙周状态的检出率并加以分析。结果 :慢性牙周炎患者牙周炎活动部位、牙周炎静止部位、轻度龈炎部位的HCMV检出率为分别为 3 8.7%、14 .5 %、12 .9% ;HCMV在牙周炎活动部位的检出率高于牙周炎静止期部位 (P <0 .0 5 ) ,以及轻度龈炎部位 (P <0 .0 5 ) ;牙周炎静止部位与轻度龈炎部位的HCMV检出率的差异无统计学意义 (P >0 .0 5 )。结论 :提示HCMV感染与慢性牙周炎及其活动性的有一定的相关性。Objective:To study the relationship between human cytome ga lovirus (HCMV) and chronic periodontitis. Methods: In 62 chronic periodontitis patients (27 male and 35 female), subgingival samples were colle cted from active periodontitis site,quiescent periodontitis site, and initial g ingivitis site of each subjects. Human cytomegalovirus was detected by nested - polymerase chain reaction method. Results: HCMV detectable ratio in the active periodontitis sites was significantly higher than that in the qui escent sites (38.7% VS 14.5%, P<0.05) and than that in the initial gingiviti s sites (38.7% VS 12.9%, P<0.05). There was no significant difference in HCM V detectable rate between quiescent periodontitis sites and initial gingivitis s ites (P>0.05).Conclusion:HCMV infection may be associated wi th the activity of chronic periodontitis.

关 键 词：人类巨细胞病毒 菌斑 慢性牙周炎 

分 类 号：R781.4[医药卫生—口腔医学]