荧光定量PCR法检测外周血淋巴细胞中EB病毒DNA含量对鼻咽癌诊断及预后的价值  

Clinical application of fluoroquantitative analysis of lymphocyte Epstein-Barr virus DNA by PCR to diagnosis and prediction of prognosis of nasopharyngeal carcinoma.

作　　者：田绍东[1] 唐孝亮[1] 孙延亮[1] 肖拥军[1] 

机构地区：[1]南华大学附属怀化医院,湖南怀化418000

出　　处：《现代肿瘤医学》2005年第1期44-45,共2页Journal of Modern Oncology

摘　　要：目的　评价外周血淋巴细胞中EB病毒DNA含量对鼻咽癌诊断及预后的价值。方法　采用荧光定量PCR方法检测 52例鼻咽癌患者和 127例非鼻咽癌患者外周血淋巴细胞中EB病毒DNA含量。结果　鼻咽癌患者中EB病毒DNA的阳性率为 55 76% (29 /52),对照组阳性率为 8 66% (11 /127),阳性率差异有显著性P<0. 01。早期(Ⅰ、Ⅱ期)鼻咽癌患者EB病毒DNA含量与晚期 (Ⅲ、Ⅳ期 )比较有显著性差异(P<0 05)。结论　荧光定量PCR方法检测外周血淋巴细胞中EB病毒DNA含量在鼻咽癌的诊断上有极高价值,可作为判断疗效及监测病情的一种有效手段。Objective To evaluate the ro1e of Epstein-Barr virus(EBV)DNA in peripheral lymphocyte in diagnosis and predication of patients with nasopharyngeal carcinoma (NPC). Peripheral lymphocyte EBV-DNA in 52 cases of NPC and 127 cases of non-NPC were analysed by fluoroquantitative Polymerase Chain Reaction(PCR).Results The positive rate of lymphocyte EBV-DNA in NPC group was 55.76%(29/52)and 8.66%(11/127) in the contro1 group,the percent of postive in the fomer was significant higher than the later,P<0.01.EBV-DNA concentration of patient with NPC in early stage(phase I、Ⅱ)was lower than 1ater stage(Ⅲ、Ⅳ),P<0.05.Conclusion Fluoroquantitative analysis of peripheral lymphocyte EBV-DNA may be a valuble technique for diagnosing patients with NPC and predicting the prognosis.

关 键 词：EB病毒 DNA 鼻咽癌 聚合酶链反应 诊断及预后 

分 类 号：R739.63[医药卫生—肿瘤]