开角型青光眼患者外周血中IL-4、IL-6、IL-12含量的研究  

IL-4,IL-6 and IL-12 levels in patients with open-angle glaucoma

作　　者：黄萍[1] 张纯[1] 

机构地区：[1]北京大学附属第三医院眼科,北京市100083

出　　处：《眼科新进展》2005年第1期41-42,共2页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助 (编号 :30 371 50 4 )

摘　　要：目的　观察开角型青光眼患者与正常人外周血中IL 4、IL 6、IL 12的水平差异。方法　青光眼组 15例 ,正常对照组 15例。 2组年龄无显著性差异。分别取外周血 3mL ,采用双抗体夹心酶联免疫吸附法 (ABC ELISA)方法分别测定IL 4、IL 6、IL 12含量。结果　IL 4含量 :正常对照组为 (2 4 .0 85± 6 .0 88)pg·mL-1;青光眼组为 (2 8.348± 2 0 .131)pg·mL-1,P =0 .4 93,2组间无显著性差异。IL 6含量 :正常对照组为 (7.131± 8.4 15 ) pg·mL-1,青光眼组为 (1.383± 2 30 3)pg·mL-1,P =0 .0 2 1,具有显著性差异。IL 12含量 :正常对照组为 (19.4 0 0± 4 .85 0 )pg·mL-1;青光眼组为 (12 2 15± 4 .12 1) pg·mL-1,P =0 .0 0 0 ,2组相比具有高度统计学差异。结论　开角型青光眼患者外周血中IL 6 ,IL 12的含量显著低于正常人。开角型青光眼患者机体内的细胞免疫水平不同于正常人 ,提示免疫因素可能在视神经损害机制中起重要作用。Objective To analyze the level difference of the cytokine IL-4,IL-6 and IL-12 in peripheral blood between patients with open angle glaucoma and normal subjects.Methods In this study,15 patients and 15 controls were included.IL-4,IL-6,IL-12 were assayed by Enzyme-linked immunosorbent assay.Results IL-4,IL-6,IL-12 concentrations in glaucomatous and control groups were (28.384±20.131) pg·mL -1,(1.383±2.303)pg·mL -1,(12.215±4.121) pg·mL -1and (24.085±6.088) pg·mL -1,7.131±8 415 pg·mL -1 and (19.400±4.850) pg·mL -1 respecttively.The mean concentrations of IL -6 and IL -12 in glaucoma group were higher than that in control group(P=0 021,0.000).There were no significant difference in IL-4 concentrations between two groups(P=0 493).Conclusion The levels of IL-6,IL-12 in peripheral blood of patients with open-angle glaucoma were lower observably than that of normal subjects.The alteration of the cellular immune system in patients with open-angle glaucoma supports our hypothesis that the immune system may play an important role in the initiation and sustainment of glaucomatous optic neuropathy in some patients.

关 键 词：青光眼 免疫 白细胞介素 

分 类 号：R775[医药卫生—眼科]