硫化氢体系在大鼠低氧性肺动脉平滑肌细胞增殖中的意义  

The Role of Hydrogen Sulfide System on Pulmonary Artery Smooth Muscle Cells Cult ured of Rats in Hypoxia Condition

作　　者：张春雨[1] 丛柏林[2] 宫丽敏[1] 杜军保[1] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]承德医学院,河北承德067000

出　　处：《实用儿科临床杂志》2005年第2期140-141,i002,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金资助项目(30271373);国家重点基础研究发展规划项目(973);教育部高等学校博士学科点专项基金资助项目(20020001063)

摘　　要：目的　探讨低氧对肺动脉平滑肌细胞中胱硫醚 γ 裂解酶 (CSE)基因表达的影响和硫化氢对肺动脉平滑肌细胞增殖的调节作用。方法　人工培养大鼠的肺动脉平滑肌细胞 ,实验分为 1.对照组 ;2 .硫氢化钠 (NaHS)组 :10 - 4mol/LNaHS处理 2 4h ;3 .低氧组 :氧浓度 1%以下 2 4h ;4.低氧 +NaHS组 :低氧 2 4h ,10 - 4mol/LNaHS。每组各 6孔细胞。用3H TdR掺入估计细胞增殖 ,用定量竞争性RT PCR方法测定CSEmRNA含量 ,评价CSE基因表达。结果　低氧组平滑肌细胞中的CSEmRNA含量较对照组减少 67% (P <0 .0 1)。低氧组细胞的3H TdR掺入较对照组增加 66% (P <0 .0 1) ;NaHS组细胞的3H TdR掺入较对照组减少 3 7%(P <0 .0 1) ;低氧 +NaHS组的3H TdR掺入较低氧组减少 2 9% (P <0 .0 1)。结论　低氧使人工培养的大鼠肺动脉平滑肌细胞中CSE基因表达下调 ,硫化氢对大鼠肺动脉平滑肌细胞增殖有抑制作用。Objective To explore the influence of hypoxia on cystathionine gamma lyase (CSE) gene expr ession of rat cultured pulmonary artery smooth muscle cells (PASMCs) and the mod ulatory effect of hydrogen sulfide on PASMCs proliferation.Methods Rat′s cultured PASMCs were randomly divided into contr ol group,NaHS group,hypoxia group and hypoxia +NaHS group. Each group contains 6 wells of cells. Hypoxic challenge was performed with oxygen concentr ation≤1%. The cell proliferation rate was measured by 3H-thymidine i ncorporation into DNA of the PASMCs. The expression of CSE mRNA was measured by quantitative competitive reverse-transcription polymerase chain reaction (RT-PCR ).Results For the PASMCs in hypoxia group, the amount of CSE mRN A was down-regulated about 67% (P<0.01) compared with control group. Compare d with control group, the 3H-thymidine incorporation was increased by 66% (P<0.01) for PASMCs in hypoxia group; compared with control group, the 3H-thymidine incorporation was decreased by 37% for PASMCs in NaHS gr oup; compared with hypoxia group,the 3H-thymidine incorporation was d ecreased by 29% for PASMCs in hypoxia+NaHS group.Conclusion Hypoxia challenge can down-regulate the CSE gene exp ression;and hydrogen sulfide can suppress the proliferation of rat′s PASMCs. J Appl Clin Pediatr,2005,20(2):140-141

关 键 词：硫化氢 低氧 肺动脉平滑肌细胞 

分 类 号：R363[医药卫生—病理学]