C2-神经酰胺诱导大鼠脑胶质瘤细胞C6的早期凋亡作用  

Induction effects of C2-ceramide on early apoptosis in rat glioma cell C6

作　　者：祁红[1] 刘艳梅[1] 吴兴军[1] 史桂英[2] 陈红专[1] 

机构地区：[1]上海第二医科大学药理学教研室,上海200025 [2]上海第二医科大学细胞生物学教研室,上海200025

出　　处：《中国临床药理学与治疗学》2004年第12期1349-1352,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：上海市教委重点学科基金资助 (№ 2 0 0 3JY0 4)

摘　　要：目的 :研究外源性C2 神经酰胺对大鼠脑胶质瘤细胞C6活力的抑制作用以及早期凋亡诱导作用。方法 :采用MTT法测定C2 神经酰胺对C6细胞活力的影响 ;倒置荧光显微系统观察细胞形态变化 ;An nexinⅤ PI双染色流式细胞术对细胞早期凋亡进行定量分析。结果 :C2 神经酰胺可显著抑制C6细胞的活力 ,IC50 为 2 .2× 10 -5mol·L-1;荧光染色可见核浓染等细胞凋亡特征形态改变 ;流式细胞术分析表明C2 神经酰胺可诱导C6细胞发生早期凋亡作用 ,且凋亡百分率呈时间及浓度依赖性 ,C2 神经酰胺 2× 10 -5mol·L-1处理 2 4h后平均早期凋亡率高达4 9 .3%。结论 :C2 神经酰胺主要通过诱导早期细胞凋亡对大鼠脑胶质瘤C6细胞发挥细胞毒作用 ,提高神经酰胺水平有望成为肿瘤化疗的新途径。AIM: To investigate the effects of exogenous cell-permeable C2-ceramide on viability and early apoptosis induction in rat glioma ce ll C6. METHODS: The effects of C2-ceramide on viability of C6 c ell after 24 h treatment were assessed by MTT test. The cellular morphology was observed by Olympus inverted reflected light fluorescence observation attachment . The early events of the apoptosis were recorded by flow cytometry analysis wit h a combination of Annexin V-FITC and propium iodide (PI) which was used for st aining cells. RESULTS: C2-ceramide significantly inhibited the cell viability of C6 cells and the IC 50 was 2.2×10 -5 mol ·L -1. The classical apoptotic morphology change was induced by C2-cera mide, and the early cell apoptosis was induced in a time and concentration-depe ndent manner. After treated with 2×10 -5 mol·L -1 C2-ceramide f or 24 h, the average apoptotic death rate of cells was about 49.3%. CONCLUSION: C2-ceramide can exert obvious cytotoxic effect in C6 cell t hrough inducing typical early apoptosis of C6 cells, and it suggest that the enh ancement of the level of ceramide might offer a novel approach to cancer chemoth erapy.

关 键 词：C2-神经酰胺 C6 ANNEXIN Ⅴ/PI 早期凋亡 

分 类 号：R966[医药卫生—药理学]