Fas/FasL和颗粒酶B在口腔扁平苔藓中的表达及意义  

Expressions of Fas/FasL and granzyme B in oral lichen planus and their significance

作　　者：沈丽佳[1,2] 阮萍[1] 谢方方[3] 赵彤[4] 

机构地区：[1]暨南大学医学院口腔医学系 [2]南方医科大学病理教研室,广东广州510515 [3]广西医科大学附属口腔医院口腔内科 [4]南方医科大学病理教研室

出　　处：《第一军医大学学报》2004年第12期1362-1366,共5页Journal of First Military Medical University

基　　金：广东省医学科研基金(A2002316)~~

摘　　要：目的探讨Fas/FasL、颗粒酶B与口腔扁平苔藓(orallichenplanus,OLP)中的细胞凋亡及与OLP病变发生发展的关系。方法采用脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法(TUNEL法)、免疫组化技术检测50例(20例糜烂萎缩型,30例非糜烂型)OLP及10例正常口腔黏膜中Fas、FasL及颗粒酶B的表达及细胞凋亡情况。结果OLP上皮细胞的凋亡指数(95.32±28.99)比正常对照组(42.52±0.05)高(P<0.05),且OLP中凋亡上皮细胞多位于基底层或基底上层,而正常对照组的凋亡上皮细胞多位于表层及棘层。OLP固有层淋巴细胞的凋亡指数(35.12±9.89)则低于正常组(61.58±0.10)(P<0.05)。OLP固有层FasL及颗粒酶B的阳性率分别为68%、68%,均高于正常对照组(P<0.05),其中糜烂萎缩型OLP固有层中淋巴细胞FasL及颗粒酶B的表达均明显高于非糜烂型OLP(P<0.05)。结论口腔扁平苔藓中同时存在角质细胞的凋亡增加及淋巴细胞凋亡的减少,Fas/FasL及颗粒酶B可能与这种凋亡的异常相关,且FasL及颗粒酶B的高表达与OLP病情发展有密切关系。Objective To define the association of the expressions of Fas/FasL and granzyme B with cell apoptosis in oral lichen planus (OLP) and progression of the disease. Method Immunohistochemical method and TUNEL were employed to study the expressions of Fas, FasL and granzyme B and cell apoptosis in 50 OLP cases (including 20 cases of atrophic-erosive OLP and 30 nonerosive OLP cases) and 10 normal oral mucosa specimens. Results Compared with the control group, the OLP cases showed increased apoptotic index (AI, 95.32±28.99) in the epithelial layer and the decreased AI (35.12±9.89) in the lamina propria (P<0.05), with the apoptotic cells confined within the basal or suprabasal cell layer instead of the superficial cell layer in the control group. The positivity rates of FasL and granzyme B expression were both 68% in the lamina propria of the OLP cases, remarkably higher than those in the normal control group (P<0.05); significantly higher rates were noted in atrophic-erosive than in nonerosive OLP cases (P<0.05). Conclusions The AI significantly differs between the epithelial layer and lamina propria in OLP cases, for which the expressions of Fas, FasL and granzyme B might be held responsible. The over-expressions of FasL and granzyme B are closely related to the progression of OLP.

关 键 词：口腔扁平苔藓 FAS/FASL 颗粒酶B 细胞凋亡 

分 类 号：R781.5[医药卫生—口腔医学]