水稻醇溶蛋白启动子驱动下的ipt基因表达及对烟草种子iPAs含量和种子重量的影响  

Effect on Transgenic Tobacco by ipt Gene Expression Which Under Control of Rice Prolamin Promoter

作　　者：申佩弘 乔越美[1] 武波[1] 

机构地区：[1]广西大学生命科学与技术学院/教育部微生物及植物遗传工程重点实验室/广西亚热带生物资源保护利用重点实验室,南宁530005

出　　处：《中国农业科学》2004年第12期1938-1941,共4页Scientia Agricultura Sinica

基　　金：教育部"高等学校青年骨干教师基金";广西"十百千人才工程"专项经费(2000206)资助项目

摘　　要：将种子特异性表达的水稻醇溶蛋白启动子P与ipt基因融合,构建了pBI121-Pi植物表达载体,通过农杆菌LBA4404介导将基因整合到烟草的基因组中,利用抗生素筛选和PCR检测及序列分析,筛选出转基因的植株,成熟种子通过ELISA试剂盒检测其细胞分裂素含量,发现iPAs的含量为对照的4.17倍,种子的千粒重与对照相比增加了12.14%。An ipt gene, driven under the endosperm-specific rice prolamin promoter (P), was cloned to a plant expression vector pBI121, named pBI121-Pi. It was introduced into tobacco by A.tumefaciens mediated transformation. PCR and sequence analysis confirmed that the P-ipt has integrated into the genomes of tobacco plants. Cytokinin level in seeds was determined by ELISA kit. Results showed that the iPAs level in transgenic seeds is about 4.17 fold increase as compared with that in seeds of control plants, and the seed average weight increased by 12.14%.

关 键 词：水稻 烟草种子 醇溶蛋白 种子重量 成熟种子 植株 千粒重 含量 对照 启动子 

分 类 号：S511[农业科学—作物学] S572