膀胱移行细胞癌预后与p53基因突变关系的研究  

p53 Gene Mutation and Prognosis of Bladder Tumors

作　　者：王立波[1] 商磊珠[1] 张颖[1] 郑晓红[1] 许秀梅 

机构地区：[1]白求恩医科大学第一临床学院泌尿外科,吉林长春130021 [2]通辽市防疫站,内蒙古通辽028000

出　　处：《吉林师范大学学报（自然科学版）》2000年第4期15-17,共3页Journal of Jilin Normal University:Natural Science Edition

摘　　要：本文探讨了膀胱癌组织p53基因突变与临床分期、病理分级及预后的关系 .利用PCR -SSCP及PCR -直接循环测序技术 ,分析 70例膀胱移行癌组织p53基因突变 .结果 70例中 2 4例 ( 3 4 .3 % )发生p53基因突变 .浸润性膀胱癌 (pT2 -pT4 )组比浅表性膀胱癌 (pTa -pT1 )组、G1 -2组比G1组p53基因突变率明显增高 (P<0 .0 5) .突变阳性组与突变阴性组的 1年、3年、5年生存率分别为 63 .6%、4 0 .1 %、4 0 .1 %及 91 .8%、76.4 %、56.4 % ,阳性组较阴性组明显预后不良 (P <0 .0 5) .结论是p53基因突变可作为预测膀胱移行癌恶性进展及鉴别浸润性与浅表性膀胱癌、预后判断有用的分子生物学指标 .To investigate the connection for the p53 gene mutation and stage,grade and prognosis in tumors,polymoerase chain reaction single strand conformation polymorphism(PCR_SSCP)and PCR_direct cycle sequence was performed for analysis from exon 5 to 8 of p53 gene in 70 cases of bladder tumors.A significant differences were found for groups with stage pTa to pT1 and pT2 to pT4(p<0.05) tumors,grades Ⅰ and grades Ⅱto Ⅲ(p<0.05) cancer,and survival rates of 1,3 and 5 years were showed 63.6%、40.1%、40.1%and 91.8%、76.4%、56.4%,respectively(p<0.05),in case of with and without p53 gene mutations. Our results suggest mutation of p53 gene may be possibly useful as ideal molecular markers for discriminate the muscle_invasive(pT2-4) and superficial tumors,evlvement and prognosis in bladder tumor.

关 键 词：P53基因突变 预后判断 膀胱移行细胞癌 浅表性膀胱癌 阴性 阳性 膀胱癌组织 利用 发生 测序 

分 类 号：R737.14[医药卫生—肿瘤] R735[医药卫生—临床医学]