姜黄素与5-氟尿嘧啶联用对人结肠癌HT-29细胞增殖的影响  

Combined inhibitory effects of curcumin and 5-fluorouracil on growth of human colon carcinoma cell line HT-29

作　　者：杜伯雨[1] 姜丽平[2] 仲来福[1] 

机构地区：[1]大连医科大学卫生毒理学教研室,辽宁大连116027 [2]大连医科大学中日合作医药科学研究所,辽宁大连116027

出　　处：《中国药理学与毒理学杂志》2005年第1期49-52,共4页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的　体外观察 5 氟尿嘧啶与姜黄素联用对人结肠癌HT 2 9细胞增殖的相互作用。方法　采用MTT法观察不同浓度姜黄素、5 氟尿嘧啶单独或联合应用对HT 2 9细胞生长的抑制作用 ,并利用中效原理判定两药合用的效果。结果　两种药物单独应用时 ,对HT 2 9细胞的抑制作用呈明显的剂量效应关系 ;对于HT 2 9细胞 ,姜黄素的中位抑制浓度为(32± 11) μmol·L- 1,5 氟尿嘧啶的中位抑制浓度为(10 4 0± 4 5 6 ) μmol·L- 1。应用中效原理分析显示 ,两药在联合应用时为协同效应 ,并且在不同的药物浓度配比下呈相同的趋势。增大姜黄素的用量可在更宽的效应范围内获得两种药物的协同效应。结论姜黄素与 5 氟尿嘧啶在联合应用时的相互作用为协同效应 ,本结果对于结肠癌的治疗具有一定的临床意义。AIM To observe the combination effects of 5-f lu orouracil (5-FU) and curcumin in human colon carcinoma cell line HT-29 i n vitro. METHODS The combination effects and the interaction between curcumin and 5-FU on HT-29 cells were evaluated according to the media n-effect principle. RESULTS When curcumin or 5-FU was used alone, the resulting growth curves showed that both agents had the concentration-depe ndent inhibitory effect. The mean curcumin concentration plus or minus standard error of the means inhibiting 50% of the growth of HT-29 cells was (32±11) μm ol·L -1, and the mean 5-FU concentration inhibiting 50% of the growth of HT-29 cells was (1040±456)μmol·L -1. On median effect analysis the comb ined effects of the two agents given concurrently were synergistic. The same res ults could also be obtained when the two agents were used at different ratios. T he synergistic effect was shown in wider range when the dosage of curcumin was i ncreased. CONCLUSION The combination effects of curcumin and 5-FU given concurrently were synergistic. The results have clinical implications for the treatment of colon cancer.

关 键 词：结肠肿癌 HT 29细胞 5-氟尿嘧啶 姜黄素 协同效应 

分 类 号：R735.35[医药卫生—肿瘤]