阻断磷脂酶C-γ1信号通路对大肠癌细胞凋亡的影响  

Inhibition of phospholipase C gamma1 signaling pathway promotes apoptosis of human

作　　者：刘俊[1] 李明[1] 曾位森[1] 白晓春[1] 罗深秋[1] 

机构地区：[1]南方医科大学细胞生物学教研室,广东广州510515

出　　处：《第一军医大学学报》2005年第2期177-180,共4页Journal of First Military Medical University

基　　金：广东省自然科学基金(020015);全军医药卫生科研基金(01MB098)~~

摘　　要：目的探讨磷脂酶C-γ1(PLC-γ1)信号通路被阻断对大肠癌细胞凋亡状态的影响。方法利用U73122(1、5、10μmol/L)处理细胞以阻断PLC-γ1信号通路,光镜、电镜观察细胞形态改变,MTT法评估细胞杀伤效应,流式细胞仪分析凋亡细胞比例。结果PLC-γ1信号通路阻断后,大肠癌细胞出现了典型的凋亡形态学改变,存活细胞明显减少,流式细胞仪检测出现典型的凋亡亚二倍体峰,凋亡细胞所占比例达到半数以上。结论阻断PLC-γ1信号通路能够启动大肠癌细胞凋亡。Objective To investigate the effects of inhibiting phospholipase C gamma1 signaling pathway on the apoptosis of human colorectal carcinoma cells. Methods SW620 cells were treated with U73122 in vitro to inhibit the phospholipase C gamma1 signalling pathway and examined under light microscope and transmission electron microscope for analyzing changes in apoptotic behavior of the cells. MTT assay was used to evaluate the cell killing effects, and the percentage of apoptotic cells analyzed using flow cytometry. Results After inhibition of the phospholipase C gamma1 signaling pathway by U73122, SW620 cells exhibited obvious apoptotic morphology, the viable cells decreased dramatically, and the percentage of apoptotic cells rose to above 50%. Conclusion Inhibition of phospholipase C gamma1 signaling pathway can induce apoptosis of human colorectal carcinoma cells.

关 键 词：磷脂酶C-Γ1 信号通路 大肠肿瘤 凋亡 

分 类 号：R735.34[医药卫生—肿瘤]