哈萨克族食管癌差异表达基因的研究  

Screening and Cloning Differentially Expressed Genes of Esophageal Cancer in Hazak

作　　者：卢晓梅[1] 张月明[2] 林仁勇[1] 张亚楼[1] 阿孜古丽[1] 吴明拜[3] 张铸[3] 伊力亚尔[3] 温浩[1] 

机构地区：[1]新疆医科大学一附院中心实验室,乌鲁木齐830054 [2]新疆医科大学公共卫生学院 [3]新疆医科大学一附院胸外科

出　　处：《肿瘤防治研究》2005年第2期79-81,共3页Cancer Research on Prevention and Treatment

基　　金：新疆维吾尔自治区高校科研计划青年教师科研基金资助项目(No.XJEDV2004S14);国家自然科学基金资助项目(No.30260049)

摘　　要：目的　筛选并克隆在哈萨克族食管鳞癌与正常食管组织之间差异表达的基因。方法　(1)应用抑制性消减杂交(SSH)进行基因差异表达分析,构建哈族食管鳞癌组织和正常食管黏膜组织之间差异表达的cDNA消减文库;(2)克隆、鉴定食管癌组织特异表达的基因;(3)登录Genbank,运用 Blastn程序进行同源性分析。结果　成功构建了消减效率高的哈族食管鳞癌 cDNA消减文库,对其中 6 个克隆的插入cDNA片断进行测序,经检索Genbank表明:这些差异表达基因与跨膜受体、乳腺癌转录因子、蛋白剪接基因及染色体1、8不同区域有较高的同源性。结论　通过抑制性消减杂交技术构建了哈族食管鳞癌cDNA消减文库,并分析鉴定了部分差异表达基因,为进一步筛选鉴定食管鳞癌特异性基因及其全长克隆、功能研究等提供了依据。Objective To clone and identify the differentially expressed genes which are related to Hazak′s esophageal cancer.Methods (1)Using suppression subtractive hybridization(SSH)to construct a subtractive library containing differentially expressed genes′ fragments;(2)Cloning and identifying these special genes;(3)Homology analysis of cDNA fragments from SSH by Blastn through Genbank.Results The differentially expressed gene cDNA library for Hazak′s Esophageal cancer was successfully constructed .Through Blastn analysis,sequences of 6 positive clones showed that they were homologous with the genes related transmembrane helix receptor,breast cancer putative transcription factor, spliced protein gene,chromosome 8, clone RP11-27N21,clone RP11-301G21 on chromosome 1 published in GenBank.Conclusion The differentially expressed gene cDNA library from Hazak′s esophageal cancer was successfully constructed and it would lay the foundation for further screening full-length genes in Hazak′s esophageal cancer.

关 键 词：萨克族食管癌 抑制性消减杂交 差异表达基因 

分 类 号：R735.1[医药卫生—肿瘤]