Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS  

作　　者：潘春秀 徐秀珠 何红梅 蔡小军 张雪君 

机构地区：[1]Department of Chemistry,Zhejiang University,Hangzhou310027,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2005年第1期74-78,共5页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project(No.03111)supportedbytheFoundationofAnalysis&MeasurementofZhejiangProvince,China

摘　　要：The cis and trans isomers separation of 2-butene-1,4-diol and lafutidine were studied by HPLC on two kinds of chiral columns: (S,S)-Whelk-O 1 and ChiraSpher. The isomers of 2-butene-1,4-diol can be separated on both chiral columns while the isomers of lafutidine can only be resolved on ChiraSpher column. The influence of different type and amount of mobile phase modifier on the isomers separation was extensively studied. The resolution of cis and trans isomers of 2-butene-1,4-diol was 2.61on (S,S)-Whelk-O 1 column with hexane-ethanol (97:3, v/v) as the mobile phase. The resolution of lafutidine was 1.89 on ChiraSpher column with hexane-ethanol-THF-diethylamine (92:3:5:0.1, v/v/v/v) as the mobile phase. LC-MS methods were developed to identify the isomer peaks.The cis and trans isomers separation of 2-butene-1,4-diol and lafutidine were studied by HPLC on two kinds of chiralcolumns:(S,S)-Whelk-O 1 and ChiraSpher.The isomers of 2-butene-1,4-diol can be separated on both chiral columns while theisomers of lafutidine can only be resolved on ChiraSpher column.The influence of different type and amount of mobile phasemodifier on the isomers separation was extensively studied.The resolution of cis and trans isomers of 2-butene-1,4-diol was 2.61on(S,S)-Whelk-O 1 column with hexane-ethanol(97:3,v/v)as the mobile phase.The resolution of lafutidine was 1.89 onChiraSpher column with hexane-ethanol-THF-diethylamine(92:3:5:0.1,v/v/v/v)as the mobile phase.LC-MS methods weredeveloped to identify the isomer peaks.

关 键 词：2-butene-1 4-diol LAFUTIDINE Isomers separation (S S)-Whelk-O 1 ChiraSpher LC-MS 

分 类 号：O657.63[理学—分析化学]