壳聚糖修饰的过氧化氢生物传感器的研究  

Hydrogen peroxide biosensor with chitosan as immobilization matrix

作　　者：王存嫦[1] 阳明辉[2] 渠凤丽[2] 沈国励[2] 俞汝勤[2] 

机构地区：[1]湘南学院,湖南郴州423000 [2]湖南大学化学化工学院化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《化学传感器》2004年第4期12-16,共5页Chemical Sensors

基　　金：湖南省教育厅科研基金资助课题(02C023);湖南学院2004年重点资助课题

摘　　要：用壳聚糖固定辣根过氧化物酶(HRP),采用硫堇作为电子媒介,戊二醛作为交联剂,制备了HRP/壳聚糖/硫堇/GC电极。讨论了硫堇的固定方法,考察了该传感器在优化的实验条件下对H_2O_2的响应特性,实验结果表明,该传感器对H_2O_2表现出良好的响应特性,如响应快、稳定性好和抗干扰性能强等优点,检测范围为5.0×10^(-6)～3.0×10^(-3) mol/L。检出限为1.0×10^(-6) mol/L。电极在用于实际试样回收率的测定中,结果良好。A hydrogen peroxide biosensor has been prepared with ehitosan as immobilization matrix, thionine as electron mediator, and glutaraldehyde as cross-linking agent. The immobilization procedure of thionine has been discussed in detail. The effect of experimental conditions on the biosensor response has been investigated and response characteristic discussed. The biosensor displayed fast response, good stability and excellent anti-interference ability. The linear response of detection of the electrode to hydrogen peroxide is in the concentration range of 5.0×10^(-6)～3.0×10^(-3) mol/L with a detection limit of 1.0×10^(-6)mol/L. The electrode was applied to the determination of recoveries of real sample with satisfactory results.

关 键 词：HRP 固定方法 壳聚糖 GC 戊二醛 辣根过氧化物酶 修饰 硫堇 H2O2 实验条件 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]