酶生物传感器固定化酶载体的研究  

Carrier material of enzyme immobilization for biosensor

作　　者：王胜娥[1] 何洁[2] 袁红雁[2] 肖丹[2] 

机构地区：[1]湖南大学化学化工学院,湖南长沙410082 [2]四川大学化学工程学院,四川成都610065

出　　处：《化学传感器》2004年第4期30-35,共6页Chemical Sensors

摘　　要：选用具有生物亲合性的蛋壳膜作载体,采用戊二醛为交联剂的交联法对葡萄糖氧化酶进行固定化,与用蛋壳膜作支撑体的溶胶-凝胶法固定化酶作比较。利用基于氧电极的葡萄糖氧化酶传感器模型,着重研究了不同条件下固定化酶的活性,发现蛋壳膜法固定化葡萄糖氧化酶的活性较高,证明了蛋壳膜是一种很好的固定化酶的载体材料。Employing eggshell membrane as a carrier and glutaraldehyde solution as a cross-linking agent, the glucose-oxidase enzyme was immobilized. By the model of glucose-oxidase biosensor based on oxygen electrode, the glucose-oxidase enzyme activity was studied, and the resuhs were compared with those of the Sol-Gel immobilization method which used eggshell membrane as platform. The results show that the eggshell membrane method has better enzyme activity than the Sol-Gel method. Eggshell membrane was proved to be a good carrier material for enzyme immobilization.

关 键 词：固定化酶载体 支撑体 交联剂 膜法 活性 研究 载体材料 蛋壳膜 葡萄糖氧化酶 用具 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]