三种检测扎幌样病毒方法的比较分析  

Comparative analysis of three methods for detection of Sapporo-like virus.

作　　者：刘翼[1] 戴迎春[1] 李建栋[1] 詹惠春[1] 聂军[1] 

机构地区：[1]南方医科大学流行病学教研室,广东广州510515

出　　处：《中国热带医学》2005年第1期7-8,52,共3页China Tropical Medicine

基　　金：广东省医学科研基金(A2003420)

摘　　要：目的 比较三种常用检测扎幌样病毒的方法,以选取适合我国标本的较优检测方法。 方法 对收集到的169份粪便分别采用三种逆转录聚合酶链反应(RT-PCR)方法进行检测,扩增产物进行测序分析。 结果 方法A、B、C检测阳性标本数分别为3份(排出诺瓦克病毒阳性标本后)、4份、1份。4份PCR产物测序分析只有1份为扎幌样病毒,其余为轮状病毒。 结论 方法C特异性最好,即半套式RT-PCR优于其它二种RT-PCR方法。Objective To compare three methods for detection of Sapporo-like virus and selection of better one. Methods Stool specimens were tested by three RT-PCR methods, respectively, and amplified products were sequenced and analyzed. Results The number of positive specimens was 3,4 and 1 by the three methods,respectively.Only one can be determined to be Sapporo-like virus in four sequenlially amplified products,and the other three were rotavirus. Conclusion The third method or the semi-nested RT-PCR is better for detection of Sapporo-like virus.

关 键 词：扎幌样病毒 测序分析 阳性标本 逆转录聚合酶链反应 轮状病毒 RT-PCR方法 套式RT-PCR 扩增产物 粪便 排出 

分 类 号：R511[医药卫生—内科学] R373.2[医药卫生—临床医学]