流式细胞学DNA分析在良、恶性涎腺多形性腺瘤中的应用价值  

Applicance of flow cytometric DNA analysis in the benign and malignant pleomorphic adenomas of salivary gland

作　　者：郑书深[1] 王洁[1] 董福生[1] 董玉英[1] 侯亚丽[1] 

机构地区：[1]河北医科大学口腔医院,河北石家庄050017

出　　处：《山东医药》2005年第1期17-18,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目 (No.3 0 2 714 2 2 );河北省自然科学基金资助项目 (No.C2 0 0 40 0 0 62 4)

摘　　要：目的　探讨流式细胞学 DNA分析 (FCM)对良、恶性涎腺多形性腺瘤的临床价值。方法　采用流式细胞仪测定 5 0例良性涎腺多形性腺瘤、18例恶性涎腺多形性腺瘤和 10例正常涎腺石蜡包埋组织的 DNA倍体及细胞增殖活性 ,分析其变化与预后的关系。结果　正常涎腺组织 DNA皆为二倍体 ;良性涎腺多形性腺瘤 DNA二倍体率为 84 % (42例 ) ,异倍体率 16 % (8例 ) ;恶性涎腺多形性腺瘤分别为 6 1% (11例 )、39% (7例 ) ,与良性者比较有显著性差异 (P<0 .0 5 )。结论　 FCM诊断良、恶性涎腺多形性腺瘤具有一定价值 ,其应用应与形态学检查相结合。Objective To investigate the clinical value of flow cytometric(FCM) DNA analysis in the benign and malignant pleomorphic adenomas of salivary gland.Methods 50 cases benign pleomorphic adenomas of salivary gland and 18 cases malignant pleomorphic adenomas of salivary gland and 10 cases normal salivary gland were studied by FCM.DNA ploid rate and proliferation of cells were analyzed.Results The FCM DNA diploid rate of the benign pleomorphic adenomas of salivary gland was 84%,and aneuploidy rate was 16%.The FCM DNA diploid rate of the malignant pleomorphic adenomas of salivary gland was 61%,and aneuploidy rate was 39%.There was statistically significant difference between the benign and malignant pleomorphic adenomas of salivary gland.Conclusion The FCM has diagnostic value in the benign and malignant pleomorphic adenomas of salivary gland.

关 键 词：涎腺多形性腺瘤 恶性 良性 流式细胞学 正常 诊断 FCM DNA分析 二倍体 异倍体 

分 类 号：R739.87[医药卫生—肿瘤] R735[医药卫生—临床医学]