FHIT及nm23-H_1基因编码蛋白表达与胃癌临床病理生物学行为  

Protein expression of fragile histidine triad and non-metastasis 23-H_1 and their clinicopathological significance in gastric cancer

作　　者：高余佳[1] 毛晓韵[1] 吴东瑛[1] 张淑敏[1] 辛彦[1] 

机构地区：[1]中国医科大学附属一院肿瘤研究所第四研究室,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2004年第11期2534-2538,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助.No.30371607~~

摘　　要：目的:观察脆性组氨酸三联体(FHIT)基因和nm23基因在胃癌中的表达,探讨其与胃癌临床病理因素的关系. 方法:采用PV9000免疫组化二步法检测98例胃癌组织中FHIT和nm23蛋白的表达. 结果:FHIT和nm23在胃癌中表达率分别为38.8%(38/98)和33%(28/87).FHIT蛋白的缺失在胃癌中占62.1%.胃癌中FHIT的缺失与组织学类型,Lauren分型和淋巴结转移相关(P<0.05);临床病理分期愈晚,FHIT蛋白表达缺失率愈高,但无显著差异(P>0.05).nm23蛋白阳性表达与临床病理分期,淋巴结转移呈负相关(P<0.05). 结论:胃癌组织中FHIT蛋白的缺失是频发事件,FHIT可能是胃癌发生中重要的侯选抑癌基因.FHIT及nm23基因编码蛋白在胃癌中的表达与胃癌淋巴结转移密切相关,且可能共同起作用,可作为临床预测转移及估计预后的重要指标.AIM: To explore the expression of fragile histidine triad (FHIT) and non-metastasis 23-H1 (nm23-H1) and to investigate their relations with clinicopathological behaviors of gastric cancer. METHODS: PV9000 two-step immunohistochemical method was employed to detect the expression of FHIT and nm23-H1 in 98 cases of gastric cancer. RESULTS: The positive rates of FHIT and nm23-H1 expression were 38.8% (38/98) and 33% (28/87) respectively. The FHIT expression related to the histological classification, Lauren classification and lymph node metastasis of gastric cancer (P<0.05). The positive rate of FHIT protein expression became higher with the development of gastric cancer, but there was no significance (P >0.05). The nm23-H1 expression was negatively related to clinical staging and lymph node metastasis (P <0.05). CONCLUSION: FHIT may be an important candidate of tumor suppressor gene in gastric cancer. The expression of FHIT and nm23-H1 protein has close relations with lymph node metastasis in gastric cancer, and they may work syn-ergistically and can be important markers for predicting metastasis and evaluating prognosis of gastric cancer.

关 键 词：FHIT NM23-H1 基因编码蛋白 表达 胃癌 病理生物学 

分 类 号：R735.2[医药卫生—肿瘤]