E-钙黏附素和β-连环素在胰腺上皮内瘤变和胰腺癌组织中的表达  

E-cadherin and β-catenin expression in pancreatic intraepithelial neoplasia and pancreatic adenocarcinoma

作　　者：郑建明[1] 朱明华[1] 倪灿荣[1] 于观贞[1] 刘晓红[1] 林万和[1] 龚志锦[1] 

机构地区：[1]中国人民解放军第二军医大学长海医院病理科,上海市200433

出　　处：《世界华人消化杂志》2004年第11期2633-2637,共5页World Chinese Journal of Digestology

基　　金：长海医院新一轮学科建设计划<胰腺疾病基础与临床研究>基金资助项目.No.123258~~

摘　　要：目的:探讨胰腺上皮内瘤变PanIN和胰腺癌组织中E-钙黏附素(E-Cad)和β-连环素(β-Cat)异常表达的意义. 方法:回顾性研究长海医院2001-01/2003-12间外科切除和同期尸检的156例胰腺标本,并构建了组织芯片,其中含有129灶PanIN-1A,104灶PanIN-1B,22灶PanIN- 2,11灶PanIN-3和121例导管腺癌和相应癌旁组织.用EnVision免疫组化技术检测上述病变组织中E-Cad和β- Cat的表达变化,并结合临床病理资料进行相关分析. 结果:导管腺癌中E-Cad异常表达率明显高于PanINs和正常导管(64.5%,32.3%,0%),且与胰腺癌的分化程度、淋巴结转移和神经浸润密切相关(P<0.05).PanINs和导管腺癌中E-Cad胞质表达较正常导管明显增加.β-Cat的异常表达与胰腺癌淋巴结转移和神经浸润有明显相关性(P<0.05). 高级别PanINs和导管腺癌中β-Cat胞质和胞核的表达率明显高于低级别PanINs和正常导管(P<0.05).PanINs和导管腺癌中E-Cad和β-Cat表达间呈正相关性(P<0.01,P<0.05). 结论:胰腺癌和PanINs中E-Cad和β-Cat的异常改变提示他们不仅与胰腺癌的生物学行为和预后有关,而且也参与了胰腺癌的发生.AIM: To investigate the significance of abnormal E-cadherin (E-Cad) and β-catenin (β-Cat) expression in pancreatic intraepithelial neoplasia (PanIN) and pancreatic adenoc-arcinoma. METHODS: One hundred and fifty-six cases of pancreatic samples, which were surgically removed and autopsied in Changhai Hospital from January 2001 to December 2003, were retrospectively studied. Tissue microarray blocks containing 129 PanIN-1A lesions, 104 PanIN-1B lesions, 22 PanIN-2 lesions, 11 PanIN-3 lesions, and 121 cases of pancreatic ductal adenoccarcinoma and corresponding para-cancerous tissues were constructed. EnVision method of immunohistochemistry was used to detect the E-Cad and p-Cat expression in the above tissues. The correlation between the abnormal E-Cad, p-Cat expression and clini copathological parameters was analyzed. RESULTS: The rate of E-Cad abnormal expression was significantly higher in ductal adenocarcinomas than that in PanIN lesions and normal ducts (64.5% vs 32.3%, 64.5% vs 0%, P<0.05), and the rate of E-Cad abnormal expression was in significant relation to differentiation, lymph node metastasis and perineural invasion of pancreatic adeno carcinoma (P <0.05). There was remarkably increase of the E-Cad cytoplasmic expression in PanIN lesions and ductal adenocarcinomas compared with that in normal ducts (21.8%, 34.7% vs 1.5%; P<0.05). The rate of β-Cat abnormal expression was found to relate to lymph node metastasis and perineural invasion of pancreatic ad enocarcinoma (P <0.05). The expression of β-Cat cytoplasm and/or nucleus was significant in high-grade PanIN lesions (27.3%) and ductal adenocarcinomas (29.8%) compared with those in low grade PanIN lesions and normal ducts (3.0%, 2.3%; P <0.05). There was a positive relationship between the E-Cad and β-Cat expression in PanIN lesions and ductal adenocarcinomas (P<0.01, P<0.05). CONCLUSION: There was aberration in the expression of the E-Cad and β-Cat in PanIN lesions and ductal adenocarcinomas, suggesting that E-Cad and β-Cat not only relate to t

关 键 词：E-钙黏附素 Β-连环素 胰腺上皮内瘤变 胰腺癌 癌组织 表达 

分 类 号：R735.9[医药卫生—肿瘤]