浆膜腔积液转移性肺腺癌细胞中TTF-1的表达  

Expression of thyroid transcription factor-1 on metastatic pulmonary adenocarcinoma cells in serous effusions

作　　者：苏学英[1] 蒋莉莉[1] 李甘地[1] 谢斌[1] 江映红[1] 

机构地区：[1]四川大学华西医院病理科,成都610041

出　　处：《临床与实验病理学杂志》2004年第6期706-708,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的　探讨甲状腺转录因子 1(TTF 1)在浆膜腔积液肺腺癌细胞中的表达 ,为肺腺癌的诊断和鉴别诊断提供新的依据。方法　选用浆膜腔积液转移性腺癌共 6 0例 (胸水 4 0例 ,腹水 17例 ,心包积液 3例 )。经组织学或结合临床资料证实的肺腺癌 36例 ,泌尿生殖道腺癌 14例 ,胃肠道腺癌 8例 ,乳腺癌 2例。每例均制备HE染色的涂片和离心沉渣经琼脂和石蜡包埋而成的细胞块 ,并用细胞块切片作TTF 1免疫细胞化学染色。结果　 36例肺腺癌中有 2 6例表达TTF 1,2 4例肺外腺癌中只有 2例表达TTF 1,其敏感性为 72 2 % ,特异性为 91 7%。结论　TTF 1在浆膜腔积液肺腺癌具有较高的敏感性和特异性 ,在排除甲状腺癌的可能性后 ,TTFPurpose To analyze the expression of thyroid transcription factor-1 (TTF-1) in the metastatic pulmonary adenocarcinoma cells in serous effusions. Methods A total of 60 cases of effusion cytology were evaluated. The primary sites of origin were confirmed clinically or histologically in all patients. There were 36 adenocarcinomas from the lung, 14 adenocarcinomas from the genitourinary tract, 8 adenocarcinomas from the gastrointestinal tract and 2 adenocarcinomas from the breast. HE stained smear and cell block were prepared for each case. Immunocytochemical study was performed on the cell block sections. Results TTF-1 expressed in 26 lung adenocarcinomas and 2 of extrapulmonary adenocarcinomas, and the sensitivity was 72.2%, specificity was 91.7%. Conclusions TTF-1 is a sensitive and specific marker for adenocarcinomas of the lung in the serous effusions. The expression of TTF-1 strongly suggests that the primary site of adenocarcinoma is lung if the thyroid carcinoma could be excluded.

关 键 词：肺肿瘤 甲状腺转录因子-1 腺癌 浆膜腔积液 免疫细胞化学 细胞块 

分 类 号：R734.2[医药卫生—肿瘤]