紫杆柽柳与其它物种脂质转运蛋白基因编码蛋白的同源性比较  

Comparison of the Homology of Proteins Encoded by Lipid Transfer Protein (LTP) Genes from Tamarix androssowii and Other Species

作　　者：王玉成[1] 禇延广 姜静[1] 杨传平[1] 刘桂丰[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《植物生理学通讯》2005年第1期14-18,共5页Plant Physiology Communications

基　　金：国家转基因植物研究与产业化专项(JY03A18);国家重点基础研究发展规划("973")项目(G1999016000)

摘　　要：根据从柽柳cDNA文库克隆获得的脂质转运蛋白(LTP)的部分序列,用RACE技术克隆出其全长cDNA序列。基因的5'非翻译区96bp,3'非翻译区222bp,开放阅读框285bp,编码94个氨基酸,预计蛋白的分子量为9.9kD,等电点为8.02。此基因有8个位置保守的Cys残基及26个氨基酸的信号肽,为典型的植物脂质转运蛋白基因。其基因序列数据库(GenBank)登录号为AY574218(基因)和AAS79106(蛋白)。Based on the partial sequence of a lipid transfer protein (LTP) gene acquired from a clone in Tamarix androssowii cDNA library, the LTP gene was cloned by using rapid amplification of cDNA end (RACE) technology. The acquired gene was 603 bp in length, including 96 bp of 5' untranslated region, 222 bp of 3' untranslated region, and an open reading frame (ORF) of 285 bp. The ORF region encoded a peptide of 94 deduced amino acid residues. The deduced molecular weight of protein was 9.9 kD and its isoelectric point was 8.02. This gene contained 8 cysteine residues that were conserved in amino acid sequence, and had a signal peptide with 26 amino acids. It was a typical plant LTP gene. The gene was accepted by GenBank, the accession numbers were AY574218(gene) and AAS79106(protein).

关 键 词：转运蛋白 物种 信号肽 克隆 基因 RACE技术 开放阅读框 柽柳 同源性比较 氨基酸 

分 类 号：S793.5[农业科学—林木遗传育种]