人脑梗死周围组织星形胶质细胞反应与微血管相关性的研究  

Study on reaction of astrocytes and its correlation with microvessels of human brain and surrounding areas after cerebral infarction

作　　者：李光伟[1] 戚基萍[1] 陈艳昕[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院病理科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2005年第1期7-9,共3页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目 ( 3 0 2 70 480 );黑龙江省自然科学基金资助项目 (D0 2 0 3 )

摘　　要：目的　观察人脑梗死灶周围组织GFAP、CD31的表达 ,探索脑梗死灶周围组织星形胶质细胞的反应及与微血管的相关性。方法　采用HE染色观察星形胶质细胞和微血管的变化 ,免疫组化技术 (两步法 )检测胶质纤维酸性蛋白 (GFAP)和CD31在星形胶质细胞和微血管的表达。结果　脑梗死 8h其周围组织GFAP表达开始增加 (P <0 .0 1) ,4～ 5d表达明显增强 (P <0 .0 1) ,8～ 15d表达达到高峰 (P <0 .0 1) ,以后有所回落 ,19d时仍高于对照组 (P <0 .0 1) ;CD31阳性反应在脑梗死 8h开始增加 ,4～ 5d其阳性表达数量增多 (P <0 .0 1) ,血管扩张 ,8～ 15d达高峰 (P <0 .0 1) ,见大量新生血管 ,19d时仍比对照组高 (P <0 .0 1)。GFAP与CD31消长规律一致 (Pearsonr =0 .9930 ,P <0 .0 1,R2 =0 .986 0 )。结论　脑梗死后 15d内其周围组织星形胶质细胞增生活跃 ,并可能促进微血管重建与扩张 。Objective To observe expression of GFAP and CD31,research into the reaction of astrocytes and the correlation with microvessels in the surrounding areas after cerebral infarction in human.Methods HE and immunohistochemistry were used to observe changes of glial fibrillary acidic protein (GFAP)and CD31 in the surrounding areas after cerebral infarction in human.Results The expressions of GFAP and CD31 increased in 8h(P<0.01)and reached the peak in 8～15 days(P<0.01),their expressions had increasing trend within 15 days after cerebral infarction in human,after 15 days the expressions of GFAP and CD31 decreased and was higher than that in control group,the expression between GFAP and CD31 showed positive correlation(Pearson r=0.9930,P<0.01,R 2=0.9860).Conclusion Within 15 days after cerebral infarction astrocytes abnormally activated and possibly encourage microvessels to proliferate and dilatation,which suggests that astrocytes and microvessels play an important role in the process of injury and repair.

关 键 词：脑梗死 星形胶质细胞 微血管 

分 类 号：R743.33[医药卫生—神经病学与精神病学] R361.3[医药卫生—临床医学]