碱性成纤维细胞生长因子及WTp53在大鼠视网膜缺血再灌注损伤中的作用  

Effect of basic fibroblast growth factor and wild type p53 on the retina ischemia reperfusion injury of rats

作　　者：王红云[1] 牛膺筠[1] 袁春燕[1] 高云霞[1] 丁玉枝[1] 

机构地区：[1]青岛大学医学院附属医院眼科,山东省青岛市266003

出　　处：《中国临床康复》2005年第6期140-141,i006,共3页Chinese Journal of Clinical Rehabilitation

基　　金：山东省教委基金资助项目(J00K53)~~

摘　　要：目的:探讨碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)在大鼠视网膜缺血再灌注损伤中的作用及其机制。方法:随机将28只大鼠分为正常组和手术组,其中手术组大鼠左眼为缺血再灌注组,右眼为治疗组(玻璃体腔内注射bFGF),观察再灌注后不同时间段1,6,12,24,48,72h视网膜组织中细胞凋亡情况及WTp53的表达变化。前房加压法制作大鼠视网膜缺血再灌注损伤模型。应用末端脱氧核酸转移酶介导的脱氧三磷酸尿苷缺口末端标记(TUNEL)法检测视网膜组织中凋亡细胞数,免疫组织化学SABC法检测视网膜组织中WTp53蛋白的表达。结果:视网膜神经细胞的凋亡出现于再灌注后6h,并逐渐递增,24h达到高峰,48h开始下降,72h组不明显。WTp53蛋白表达改变与凋亡的神经细胞改变基本一致。治疗组各观察指标表达变化规律与缺血再灌注组基本一致,但凋亡细胞数目在6～48h犤6,12,24,48h分别为(358.3±43.4),(859.5±12.0),(1006.5±49.4),(695.7±72.2)个/mm2犦明显低于缺血再灌注组犤(444.8±89.7),(1053.0±96.1),(1254.0±164.8),(891.0±87.2)个/mm2犦(t=2.9131～4.299,P均<0.05);WTp53表达在6～48h缺血再灌注组显著下降(t=3.342～4.781,P<0.05;t=52.586,P<0.01)。结论:WTp53参与视网膜缺血-灌注损伤,促进神经节细胞的凋亡;AIM: To investigate the role and mechanisms of basic fibroblast growth factor(bFGF) in retina ischemia reperfusion injury. METHODS: Twenty eight rats were divided into normal group and operated group randomly, and the latter was subdivided into ischemia reperfusion group (left eye) and treatment group (bFGF was injected into the vitreous cavity of the right eye). Apoptosis and wild type p53 (WTp53) expression in the retina tissue were observed 1,6,12,24,48 and 72 hours after reperfusion respectively.The rat model of experimental retina ischemia reperfusion injury was established by the method of increasing the intraocular pressure.The number of apoptotic cells were detected by using the terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling(TUNEL) method, and the expression of WTp53 was determined with immunohistochemical strept avidin biotin complex (SABC). RESULTS:The apoptotic cells in retina began to appear at 6 hours after reperfusion and increased gradually, it reached the peak at 24 hours, then began to decrease at 48 hours, and no apoptotic cells could be found at 72 hours.The changing trend of WTp53 expression was similar to that of the apoptotic cells. In the treatment group, the changing trend of apoptotic cells and WTp53 expression was similar, except that the numbers of apoptotic cells at 6,12,24,48 hours after reperfusion[(358.3± 43.4),(859.5± 12.0),(1 006.5± 49.4),(695.7± 72.2)/mm2] were obviously less than those in the ischemia/reperfusion gorup [(444.8± 89.7),(1 053.0± 96.1), (1 254.0 ± 164.8), (891.0± 87.2)/mm2] (t=2.913 1 to 4.299, P < 0.05).The expression of WTp53 at 6 to 48 hours in the ischemia/reperfusion group descended significantly (t=3.342 to 4.781,P< 0.05;t=52.586,P< 0.01). CONCLUSION: WTp53 plays a part in retina ischemia reperfusion injury, and promotes the apoptosis of ganglion cell; bFGF can inhibit the increase of WTp53 expression, reduce the apoptosis of ganglion cells, and thus has a therapeutical effect on retina ischemia reperfusion

关 键 词：WTP53 视网膜缺血再灌注损伤 大鼠 表达 凋亡细胞 碱性成纤维细胞生长因子 治疗组 核酸 转移酶 FGF 

分 类 号：R735.1[医药卫生—肿瘤] R774[医药卫生—临床医学]