福氏2a志贺菌肠毒素ShET1/ShET2基因分型在菌痢快速诊断中的应用  

Application of ShET-1/ShET-2 genotyping of Shigella flexneri 2a for the rapid diagnosis of bacillary dysentery

作　　者：郝加虎[1] 叶冬青[1] 王红[2] 黄芬[1] 方益荣[1] 张国庆[1] 钟文龙[3] 夏桂枝[2] 俞守义[2] 

机构地区：[1]安徽医科大学公共卫生学院流行病与卫生统计学系,合肥230032 [2]第一军医大学流行病学教研室 [3]广东省江门市卫生防疫站

出　　处：《中国人兽共患病杂志》2005年第2期100-104,共5页Chinese Journal of Zoonoses

摘　　要：目的对福氏2a志贺菌肠毒素ShET1(Shigellaenterotoxin1)和肠毒素ShET2(Shigellaenterotoxin2)进行基因分型,提高菌痢爆发流行时同源克隆的鉴定分析水平。方法对93株分离自不同地区,不同时间的福氏2a志贺菌用PCR法检测志贺菌肠毒素ShET1/ShET2基因,进行基因分型和同源克隆鉴定。结果93株福氏2a志贺菌按志贺菌肠毒素ShET1/ShET2基因可分为4种基因型,即12株ShET1(-)/ShET2(+),14株ShET1(+)/ShET2(-),59株ShET1(+)/ShET2(+),8株ShET1(-)/ShET2(-)。93株福氏2a志贺菌ShET1检出率为89.24%(83/93),ShET2为65.59%(61/93)。二者至少有一种基因被检出的检出率为91.39%(85/93)。结论福氏2a志贺菌的快速诊断可应用ShET1、ShET2双基因PCR检测,具有较高的敏感性与特异性。在应用多生物学标志进行福氏2a志贺菌同源克隆鉴定系统研究时,肠毒素ShET1/ShET2基因PCR分析是不可或缺的分析指标。To investigate the characteristics of genotyping of Shigella entrotoxin-1 and-2 (ShET-1 and ShET-2) of Shigella flexneri 2a in order to improve the identification level for the homologous clones of this organism during outbreak of bacillary dysentery, the genes encoding for ShET-1 and ShET-2 from 93 strains of S.flexneri 2a isolated from different areas and different times were amplified by PCR and the genotyping as well as the homologous clone identification were performed thereafter. It was found that 4 different genotypes were discovered in these 93 strains, in which 12 strains were ShET-1(-)/ShET-2(+) type, 14 strains were ShET-1(+)/ShET-2(-); 59 strains were ShET-1(+)/ShET-2(+) and 8 strains were ShET-1(-)/ShET-2(-). The detection rate for ShET-1 and-2 were 89.24%(83/93) and 65.59%(61/93) respectively. The positive rate by which at least one gene was detected was ~91.39% (85/93). It is concluded that the PCR analysis for ShET-1 and-2 genes is a simple, rapid method for the diagnosis of S.flexneri 2a infection and it may be necessary index for the identification of the homologous clones of S.flexneri by using multiple biomarkers.

关 键 词：福氏2A志贺菌 分子流行病学 志贺菌肠毒素 

分 类 号：R378.2[医药卫生—病原生物学]