脂联素受体在胰岛细胞表达,脂联素促进胰岛素的分泌  

Adiponectin receptors are expressed on islet cells and adiponectin stimulates insulin secretion

作　　者：顾卫琼[1] 陈名道[1] 顾元骏[1] 唐金凤[1] 孙首悦[1] 刘长勤[1] 洪洁[1] 张翼飞[1] 李小英[1] 宁光[1] 

机构地区：[1]上海第二医科大学附属瑞金医院内分泌代谢病科,上海市内分泌代谢临床医学中心,上海内分泌代谢病研究所,200025

出　　处：《中华内分泌代谢杂志》2005年第1期68-70,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：上海市卫生局攻关课题 ( 2001ZD002 );国家自然基金(30270625);上海教委曙光计划 (01SG48 );上海市教委重点学科建设经费(2001)

摘　　要：目的检测脂联素受体(AR)在大鼠胰岛细胞的表达和脂联素对体外胰岛细胞分泌胰岛素的影响.方法 RT-PCR和免疫细胞化学方法检测AR1、AR2的mRNA和蛋白表达;并在体外用脂联素(100 μg/L)和不同浓度葡萄糖(3.3,5.6,16.7 mmol/L)处理胰岛细胞,放免法测定上清液的胰岛素浓度.结果 RT-PCR扩增出胰岛AR1和AR2基因,并经直接和亚克隆测序证实;胰岛免疫细胞化学荧光染色AR1和AR2呈阳性;经脂联素处理后的胰岛细胞,在高糖(16.7 mmol/)培养6～24 h,其胰岛素分泌持续增加(均P＜0.05).结论胰岛细胞上存在AR1和AR2,以前者为主.在高糖情况下,一定浓度的脂联素可在体外促进胰岛细胞的胰岛素分泌和释放.Objective To assay the expression of adiponectin receptor (AR) on rat islet cells and the effect of adiponectin on insulin secretion of islets in vitro. Methods The gene expression of AR1 or AR2 on islets was confirmed by both RT PCR and immunocytochemistry. Rat islets were isolated and incubated with adiponectin (100 μg/L) and graded various glucose concentrations (3.3, 5.6, 16.7 mmol/L) for 2, 6 and 24 h, respectively. The insulin levels in supernatant were measured by RIA.. Results AR1 and AR2 (esp. AR1) were expressed in rat islets testified by RT PCR andimmunocytochemistry.WhenincubatedinDMEM medium with 16.7 mmol/L glucose for 6 h and 24 h, insulin secretion of the islet cells undergoing adiponectin (100 μg/L) treatment was continuously increased and maintained at a higher level during 24 h (both P<0.05). Conclusion AR1 (predominant) and AR2 are expressed on islet cells. When isletes are incubated with high glucose concentration (16.7 mmol/L), adiponectin enhances insulin secretion of islets in vitro.

关 键 词：胰岛细胞 AR 体外 脂联素受体 胰岛素 表达 分泌 亚克隆 RNA 测序 

分 类 号：R587[医药卫生—内分泌]