人口腔黏膜癌变不同阶段端粒酶逆转录酶及p53蛋白的表达  

The expression of hTERT and p53 protein in pre-malignant and malignant lesions of human oral mucosa

作　　者：吴国英[1] 朱玲[1] 冯振卿[2] 彭韬[2] 祁兵[1] 

机构地区：[1]南京医科大学口腔医学院口腔内科,江苏南京210029 [2]南京医科大学基础医学院,江苏南京210029

出　　处：《上海口腔医学》2005年第1期51-54,共4页Shanghai Journal of Stomatology

基　　金：江苏省教育厅自然科学研究基金(03KJD320148)

摘　　要：目的:探讨人端粒酶逆转录酶(humantelomerasereversetranscriptase,hTERT)mRNA和p53蛋白在口腔黏膜癌前病变癌变过程中的作用,以及两者的关系。方法:应用原位杂交和免疫组化法对口腔黏膜单纯增生(HP)9例、轻度异常增生(LD)11例、中度异常增生(MD)10例、重度异常增生(原位癌CIS)9例、鳞癌(SCC)11例进行hTERTmRNA及p53蛋白的检测。结果应用计算机图像分析系统分析,显著性检验采用单因素方差分析(one-wayANOVA),hTERT和p53蛋白表达的相关性应用直线相关分析。结果:口腔黏膜上皮随异常增生程度HP、LD、MD、CIS到SCC,hTERT和p53蛋白的阳性细胞面积指数及光密度(OD)值均逐渐上升,SCC时达到高峰;且随着细胞异常增生程度的加重,hTERT阳性表达细胞从基底层向角化层发展,在高分化鳞癌中,主要分布在癌巢周边,分化较差的鳞癌则散布于整个肿瘤组织中。p53在HP及LD中几乎不表达,在CIS及SCC的表达率分别为66.67%和72.72%。直线相关分析显示无明显相关性。结论:hTERT与p53均参与口腔黏膜癌前病变癌变的发生发展过程,口腔鳞癌是由多基因参与的疾病。PURPOSE: To investigate the roles of hTERT and p53 protein in malignant changes of oral mucosal precancerous lesions and the relations between hTERT and p53. METHODS: The expression of hTERTmRNA and p53 were measured using in situ hybridization and immunohistochemical assay in 9 cases of oral mucosal hyperplasia,11 cases of light dysplasia,10 cases of medium dysplasia,9 cases of carcinomas in situ and 11 cases of squamous cell carcinomas. The results were processed by medical photographic system,and analyzed statistically by one-way ANOVA. The relation to hTERT and p53 was analyzed statistically by Pearson correlations. RESULTS: It was found that from hyperplasia to light dysplasia,medium dysplasia,carcinomas in situ and squamous cell carcinomas,the indexes of positive cell vessels and the value of OD increased,arriving at the apex in squamous cell carcinoma. With phenotypic progression and the degree of dysplasia,hTERT and p53 expression cells extend from basal layer to the keratinous layer. In highly-differentiated squamous cell carcinomas,expression cells were mainly located around the nests. Stained cells dispersed throughout the tumor tissue in low-differentiated squamous cell carcinomas. p53 was undetectable in hyperplasia and light dysplasia,but p53 was observed in 66.67% cases of high dysplasia and 72.72% cases of squamous cell carcinomas. There was not a significant linear correlation between hTERT and p53. CONCLUSIONS: The results suggest that activation of telomerase and p53 play a role in the process of malignant changes of oral mucosal precancerous lesions. Oral squamous cell carcinoma is a disease that has been found in association with many factors.

关 键 词：癌前病变 端粒酶逆转录酶 P53 原位分子杂交 免疫组化 

分 类 号：R739.8[医药卫生—肿瘤]