血管性痴呆小鼠海马NMDA受体免疫组织化学变化特征  

The features of N-methyl-D-aspartate receptor in neuron of hip pocampus of mice with vascular dementia by immunohistochemistry

作　　者：翟金萍[1] 陈莉[2] 吕佩源[3] 梁翠萍[3] 樊敬峰[3] 王伟斌[3] 尹昱[3] 

机构地区：[1]河北省人民医院病理科,050051 [2]河北省血液中心 [3]河北省人民医院神经内科,050051

出　　处：《疑难病杂志》2005年第1期1-3,F003,共4页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省自然科学基金资助项目(301415)

摘　　要：　目的　观测血管性痴呆小鼠海马神经元N 甲基 D 天门冬氨酸 (NMDA)受体免疫组织化学变化特征,以探讨其在血管性痴呆发病中的作用。方法　双侧颈总动脉线结,反复缺血—再灌注法制备模型,利用跳台试验和水迷宫试验观测其行为学改变,采用免疫组织化学技术观测小鼠海马神经元N 甲基 D 天门冬氨酸受体蛋白的表达变化。结果　模型组小鼠学习、记忆成绩较假手术组明显降低(P<0. 01),其海马N 甲基 D 天门冬氨酸受体蛋白表达也明显下降 (P<0. 01)。结论　血管性痴呆小鼠学习、记忆成绩下降与其海马N 甲基 D 天门冬氨酸受体蛋白低水平表达有关。Objective The characteristics of N-methyl-D-aspartate receptor in neuron of hippocampus of mice with vascular dementia (VD ) were observed for the purpose of demonstrating its function in VD pathogenesis .Methods The mice were subjected for ischemia-reperfusion repe atedly in bilateral common carotid arteries by knotting to establish the VD mode ls.The behavior abnormalities were investigated by step-down test and water m aze test,and the N-methyl-D-aspartate receptor was observed by immunohistoch emistry technique.Results The grades of learning and memory in VD mice were worse than those in sham-operation group(P<0.01),and the exp ression of N-methyl-D-aspartate receptor in VD mice was also lower than that of sham-operation group(P<0.01).Conclusion The poor grade s of learning and memory in VD mice are related to the decrease of N-methyl-D -aspartate receptor in neuron of hippocampus.

关 键 词：血管性痴呆 小鼠 海马神经元 N-甲基-D-天门冬氨酸受体 免疫组织化学 NMDA受体 表达 制备 降低 

分 类 号：R749.16[医药卫生—神经病学与精神病学]