^(131)I标记抗TPS单抗在荷人乳腺癌裸鼠体内的放射免疫显像及生物学分布  

Radioimmunoimaging and biodistribution of ^(131)I labeled anti-TPS monoclonal Antibody in nude mice bearing human breast Carcinoma

作　　者：常伟勤[1] 姜瑛[1] 刘金田[2] 周及彤[1] 

机构地区：[1]吉林大学第二医院肿瘤外科,吉林长春130041 [2]四平市中西医结合医院普外科

出　　处：《中国实验诊断学》2005年第1期119-121,共3页Chinese Journal of Laboratory Diagnosis

基　　金：长春市科技计划基金资助项目 (10 -0 82S0 6)

摘　　要：目的　研究13 1I标记的抗TPS单克隆抗体 (13 1I TPS)及正常小鼠IgG(13 1I mIgG)在荷人乳腺癌裸鼠体内的放射免疫显像及生物学分布 ,为临床应用提供依据。方法　每只荷瘤鼠尾静脉注射标记抗体 3 7MBq/ 0 1ml,于注射后2 4、4 8、96、12 0小时行SPECT ,于 4 8、96、12 0小时分批处死 ,测定肿瘤、血液、肝、肺、等重要脏器的单位重量放射性比值(T/NT)及各组织摄取百分比 (%ID/g)。结果　13 1I TPS注射后 2 4～ 12 0小时内 ,SPECT可见阳性显像 ,阳性率为 5 8% ;瘤 /血比值在 12 0小时最高 ,达 2 5 1± 0 71。13 1I mIgG注射后在 2 4～ 12 0小时内未出现放射性浓聚呈全身性分布。结论　抗TPS单克隆抗体在荷人乳腺癌裸鼠体内 ,对过度表达TPS的乳腺癌具有亲合力 ,可望用于作乳腺癌诊断及预后判断的导向载体。Objective To study the Radioimmunoimaging and distribution of 131 I labeled anti TPS monoclonal antibody and normal mice IgG in made mice bearing human breast Carcinoma and to provide the basis for clinical use. Methods 3 7 MBq/0 1 ml labeled antibody was injected into the tail vein in each respectively SPECT were measured after 24th 48th 96th 120th hours interval of injection; Radioactivity ratio of tumor end non tumor(T/NT), Percent injected dosage of every gram tissue(%ID/g) were measured after 48 96 120 hours interval to death. Results Positive of SPECT was found during 24th to 120th hour after the 131 I TPS injection, Positive rates was 58%. The peak value of T/NT Tumor blood was 2 51±0 71 at 120th hour. But non concentration of 131 I mIgG was found during 24th to 120th hour, which was distributed evenly in body. Conclusion Anti TPS monoclonal Antibody had affinity to breast carcinoma which over expressed TPS in nude mice bearing human breast Carcinoma. TPS monoclonal Antibody is prospective to be used as the direct Carrier of breast carcinoma diagnosis.

关 键 词：乳腺癌 TPS单克隆抗体 放射免疫显像 裸鼠 

分 类 号：R737.9[医药卫生—肿瘤]